Effects of Huayu Xiaopi Decoction Regulating HIF-1α/VEGF Signaling Pathway on Proliferation and Migration of AGS Cells
10.19879/j.cnki.1005-5304.202501478
- VernacularTitle:化瘀消痞汤调控HIF-1α/VEGF信号通路对AGS细胞增殖、迁移的影响
- Author:
Chongyuan GUO
1
;
Na WEI
;
Min BAI
;
Yanxia GONG
;
Weiqiang LI
;
Hairui LU
;
Yaorong AN
;
Yongqiang DUAN
Author Information
1. 甘肃中医药大学基础医学院,甘肃 兰州 730000;甘肃中医药大学甘肃省中药新产品创制工程实验室,甘肃中医药大学甘肃省中医方药挖掘与创新转化重点实验室,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
precancerous lesions of gastric cancer;
AGS cells;
Huayu Xiaopi Decoction;
HIF-1α/VEGF signaling pathway;
proliferation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(7):97-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Huayu Xiaopi Decoction on the proliferation and migration of AGS cells;To explore its mechanism in treating precancerous lesions of gastric cancer.Methods AGS cells were divided into blank group,inhibitor group and Huayu Xiaopi Decoction high-,medium-and low-dosage groups.The blank group was cultured with 15%control serum,the inhibitor group was cultured with 10 μmol/L HIF-1α inhibitor,and the Huayu Xiaopi Decoction high-,medium-and low-dosage groups were cultured with 12%,6%and 3%drug containing serum,respectively.CCK-8 method was used to detect cell survival rate,cell migration ability was detected by scratch test,immunofluorescence was used to detect the expressions of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase(MMP)-2 and MMP-9 in AGS cells,the expressions of HIF-1α,COX-2,VEGF,VEGFR2,MMP-2,MMP-9 mRNA and HIF-1α,COX-2,VEGF,VEGFR2 proteins in AGS cells were detected by RT-PCR and Western blot.Results Compared with the blank group,the cell survival rate and migration rate were significantly decreased in the inhibitor group and each dosage of Huayu Xiaopi Decoction groups(P<0.05),the expressions of PCNA,MMP-2 and MMP-9 significantly decreased in the inhibitor group and Huayu Xiaopi Decoction high-and medium-dosage groups(P<0.05),the mRNA expressions of HIF-1α,COX-2,VEGF,VEGFR2,MMP-2,MMP-9 and the protein expression of HIF-1α,COX-2,VEGF,VEGFR2 were significantly decreased(P<0.05).Conclusion Huayu Xiaopi Decoction can inhibit the proliferation and migration of AGS cells,and its mechanism is related to the regulation of the expression of key molecules in HIF-1α/VEGF signaling pathway.
