Correlation Analysis of Ubiquitin-Specific Proteases 9X and 25 with the Expression of Proliferative and Invasive Genes in Breast Cancer Tissues
10.13241/j.cnki.pmb.2025.11.003
- VernacularTitle:乳腺癌组织泛素特异性蛋白酶9X、25与增殖基因和侵袭基因表达的相关性分析
- Author:
Li REN
1
;
Su-juan DAI
;
Yong ZHANG
Author Information
1. 赣州市上犹县人民医院病理科 江西赣州 341200
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Ubiquitin-specific protease 9X;
Ubiquitin-specific protease 25;
Proliferative gene;
Invasive gene;
Clinicopathological features
- From:
Progress in Modern Biomedicine
2025;25(11):1779-1787
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the correlation between ubiquitin-specific protease 9X(USP9X),ubiquitin-specific protease 25(USP25)and expression of proliferative and invasive genes in breast cancer tissues.Methods:A total of 158 breast cancer patients who were admitted to our hospital from August 2020 to July 2023 were selected,cancer tissues and paracancer tissues were collected,the mRNA expressions of USP9X,USP25,the mRNA expressions of proliferative genes and invasive genes in the different tissues were determined by real-time fluorescence quantitative polymerase chain reaction(RT-PCR),the relationship between mRNA expressions of USP9X,USP25,mRNA expressions of proliferative genes and invasive genes were analyzed by pearson correlation,the differences in mRNA expressions of USP9X,USP25 in cancer tissues of patients with different clinicopathological features of breast cancer were compared.Results:The mRNA expressions of USP9X,USP25,proliferating genome protein Methyltransferase(EZH)2,signal transduction and transcriptional activation factor 3(STAT3),Yes associated protein 1(YAP1),gene Dickkopf-related protein 1(DKK-1),taurine up-regulated gene 1(TUG1)and NUAK family kinase 1(NUAK1)in cancer tissue were higher than those of paracancer tissues(P＜0.05).Pearson correlation test showed that,the mRNA expressions of USP9X and USP25 were positively correlated with those of EZH2,STAT3,YAP1,DKK-1,TUG1 and NUAK1(P＜0.05),the mRNA expressions of USP9X and USP25 in cancer tissue of patients with TNM stage Ⅲ was higher than that of patients with stage Ⅰ-Ⅱ,and the mRNA expressions of USP9X and USP25 in cancer tissue of patients with lymph node metastasis was higher than that of patients without metastasis,the mRNA expressions of USP9X and USP25 in cancer tissue of patients with high Ki-67 expression were higher than those of patients with low KI-67 expression(P＜0.05).Conclusion:Elevated expressions of USP9X and USP25 in breast cancer tissues,which are closely associated with patients' proliferative genes and invasive genes expression,TNM stage,lymph node metastasis and Ki-67 expression.
