Neuroinflammation-related mechanism of puerarin in improving cognitive impairment induced by alcohol exposure/withdrawal in female mice
10.12007/j.issn.0258-4646.2025.11.005
- VernacularTitle:葛根素改善酒精暴露/戒断所致雌性小鼠认知障碍的神经炎症相关机制研究
- Author:
Changlan WANG
1
;
Meijia CHENG
;
Huanhuan BIAN
;
Dan ZOU
Author Information
1. 沈阳医学院基础医学院免疫学教研室,沈阳 110001
- Publication Type:Journal Article
- Keywords:
puerarin;
learning and memory;
hippocampus formation;
pro-inflammatory cytokine
- From:
Journal of China Medical University
2025;54(11):988-994
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate how puerarin affects cognitive behavior and pro-inflammatory factors in the hippocampal formation of 52％(v/v)alcohol exposure/withdrawal in mice and reveal the underlying neuroimmunological mechanism by which puerarin improves alcohol-induced cognitive dysfunction.Methods A total of 120 adult female mice were randomly divided into control,52％alcohol,puerarin+52％alcohol,and normal saline(NS)+52％alcohol groups.The cognitive function of the animals was assessed using the Morris water maze,and the expression of target proteins in the hippocampal formation was detected using Western blotting and immunohistochemi-stry.Results The cognitive ability of mice in the puerarin+52％alcohol group was significantly higher than that of mice in the 52％alcohol and NS+52％alcohol groups.At the 20th hour after alcohol withdrawal,IL-6,TNF-α,and IL-1β expressions in the hippocampal formation of the puerarin+52％alcohol group were higher than those of the 52％and NS+52％alcohol groups.However,no significant difference could be observed in the expression levels of these cytokines between the puerarin+52％alcohol and control groups.Con-clusion In a female mouse model chronically exposed to 52％alcohol,puerarin can improve cognitive dysfunction during acute alcohol withdrawal,potentially related to the involvement of puerarin in regulating pro-inflammatory factor expressions in murine hippocampus formation.
