IGF-1 induced intervertebral disc annulus fibrosus cell aging through the inhibition of SIRT1 activity
10.3969/j.issn.1004-406X.2025.05.10
- VernacularTitle:IGF-1抑制SIRT1表达与活性介导椎间盘纤维环细胞衰老的机制研究
- Author:
Changbin LEI
1
;
Yichen BI
;
Lei ZHOU
Author Information
1. 湘南学院附属医院(临床学院) 423000郴州市;湖南省膝骨关节炎医疗医疗技术示范基地 423000郴州市;湘南学院基础医学院 423000郴州市
- Publication Type:Journal Article
- Keywords:
Annulus fibrosus cells;
IGF-1;
SIRT1;
Intervertebral disc aging
- From:
Chinese Journal of Spine and Spinal Cord
2025;35(5):522-527
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To investigate the mechanism of insulin-like growth factor 1(IGF-1)in mediating intervertebral disc cell aging through regulating the activity of the silent information regulator sirtuin 1(SIRT1).Methods:The intervertebral disc tissues of eight-week old female Spargue Dawley(SD)rats were dissected,from which annulus fibrosus(AF)cells were isolated and cultured in vitro.The effects of IGF-1 on the aging of AF cells were studied by β-galactosidase(SA-β-Gal)staining,cell proliferation assays,and cell migration experiments,in exposure to different concentrations of recombinant rat IGF-1(0,1,10,50,100ng/mL)in culture.The molecular mechanism of IGF-1 on AF cells was further explored by Western blot analysis.Results:After treatment with exogenous IGF-1 at different concentrations,SA-β-Gal staining and western blot results showed that IGF-1 promoted AF cell aging in a concentration-dependent manner.The CCK-8 analysis showed that low concentrations of IGF-1 significantly induced AF cell proliferation.Transwell assay results showed that low concentrations of IGF-1 profoundly enhanced the migratory capability of AF cells,whereas IGF-1 at high concentrations demonstrated no significant effect on AF cell migration.Western blot results showed that IGF-1 could downregulate the expression of SIRT1 and elevate the acetylation of p53.Conclusions:IGF-1 can promote AF cell aging though inhibiting SIRT1 expression and activity.
