Detection of Helicobacter pylori and Helicobacter hepaticus in patients with digestive tract diseases
10.3969/j.issn.1002-2694.2025.00.171
- VernacularTitle:消化道疾病患者感染肝螺杆菌和幽门螺杆菌的检测
- Author:
Xiaoli XU
1
;
Qize LI
;
Tingting LUO
;
Jianchao SUN
;
Qing LUO
;
Yan WANG
;
Wei ZHANG
;
Qi LIU
;
Zhenghong CHEN
Author Information
1. 贵州医科大学附属医院消化内科,贵阳 550004;贵州省微生物组与传染性疾病防控重点实验室和贵州省高等学校病原生物学特色重点实验室,贵州医科大学,贵阳 561113;贵州省消化系统疾病全省重点实验室,贵阳 550004
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Helicobacter hepaticus;
digestive tract disease;
colorectal cancer
- From:
Chinese Journal of Zoonoses
2025;41(10):1073-1080
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at investigating the presence of Helicobacter hepaticus(Hh)infection in patients with digestive tract diseases and evaluating Helicobacter pylori(Hp)infection status in patients with digestive tract cancers other than gastric cancer.Fecal samples were collected from 197 patients with digestive tract diseases at the Affiliated Cancer Hospital of Guizhou Medical Uni-versity and from 149 healthy volunteers residing in Guiyang.Hp stool antigen(HpSA)was detected with the colloidal gold method.Af-ter the extraction of fecal DNA,the Hp specific ureA gene and the Hh specific 16S rRNA gene were amplified via nested PCR,and the amplified products were subsequently confirmed through sequencing analysis.The study included 197 patients with digestive system diseases,comprising 135 cases of colorectal cancer,32 cases of chronic gastritis,22 cases of gastric cancer,5 cases of liver cancer,and 3 cases of cholangiocarcinoma.The detection rate of HpSA was 31.5%(62/197).HpSA was detected across all five disease catego-ries,and the highest detection rate was observed in patients with gastric cancer,at 50.0%(11/22),or colorectal cancer,at 24.4%(33/135).The positivity rate of Hp ureA gene PCR was 7.6%(15/197),and sequencing confirmed that the amplified products were in-deed Hp ureA gene fragments.Notably,the highest detection rate was observed in patients with colorectal cancer,at 8.9%(12/135).The positivity rate of Hh 16S rRNA gene PCR was 11.2%(22/197),and sequencing confirmed that the amplified products were in-deed Hh 16S rRNA gene fragments.Hh 16S rRNAgene presence was detected in patients with all five diseases,and the highest detec-tion rate was observed in patients with colorectal cancer,at 11.1%(15/135).Among 149 healthy volunteers,the detection rate of HpSA was 11.4%(17/149),only one case tested positive for the Hp ureA gene,and the Hh 16S rRNA gene was undetectable in all samples.In conclusion,Hh infection was detected in patients with digestive tract diseases.Beyond patients with gastric cancer,the prevalence of Hp infection was also notably high among patients with colorectal cancer,liver cancer,and cholangiocarcinoma.Further investigation is warranted to elucidate the roles of the two species of Helicobacter in the occurrence and progression of digestive tract cancers.
