The Expression of HPV E6/E7 mRNA in Situ Hybridization in High-Risk Human Papilloma Virus Typing-Negative Cervical High-Grade Squamous Intraepithelial Lesion
- VernacularTitle:原位杂交检测人乳头瘤病毒E6/E7 mRNA在高危型人乳头瘤病毒分型检测阴性子宫颈高级别鳞状上皮内病变中的表达研究
- Author:
Yuzhen LIU
1
;
Huimin FENG
;
Youzhong ZHANG
Author Information
1. 潍坊市人民医院宫颈疾病诊疗中心,山东潍坊 261000
- Publication Type:Journal Article
- Keywords:
Cervix;
High-grade squamous intraepithelial lesion;
Human papilloma virus;
Human papilloma vi-rus E6/E7 mRNA;
In situ hybridization
- From:
Journal of Practical Obstetrics and Gynecology
2025;41(5):393-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Human papilloma virus(HPV)E6/E7mRNA in situ hybridization detection technology was used to explore whether high-grade squamous intraepithelial lesion(HSIL)of cervix with negative high-risk human papilloma virus(HR-HPV)typing test was caused by HPV infection,so as to provide new insights for the accurate diagnosis and treatment of cervical HSIL.Methods:305 tissue samples were collected from patients with HSIL who underwent cervical biopsy or surgical treatment in the Department of Gynecology,Affiliated Hospital of Shandong Second Medical University,from January 2020 to October2022.Patients with negative HR-HPV genoty-ping results(15 cases)were assigned to the study group,while those with positive results(290 cases)served as the control group.RNAscope in situ hybridization was used to detect the expression of HPV E6/E7 mRNA in the two groups,and the expression of HPV E6/E7 mRNA was analyzed by semi-quantitative scoring of staining inten-sity.Immunohistochemistry(IHC)was used to detect the expression of p16 and Ki-67.Results:①Among 305 pa-tients with HSIL,15 patients had negative HR-HPV typing test,with an incidence of 4.9％(15/305).②73.3％(11/15)of the study group showed positive expression of HPV E6/E7 mRNA,which was statistically significant compared with 100.0％(290/290)of the control group(P＜0.001).③The positive expression rate of p16 in the study group was significantly lower than that in the control group(93.3％vs.100.0％,P=0.049),while there was no significant difference in the positive expression rate of Ki-67 between the two groups(100.0％vs.100.0％).④In the samples with histopathological type cervical intraepithelial neoplasia(CIN)2,the overall score of HPV E6/E7 mRNA in the study group was significantly lower than that in the control group,which was statistically significant(P=0.004).In CIN3 samples,although the overall score of HPV E6/E7 mRNA in the study group was lower than that in the control group,there was no statistically significant difference(P=0.158).Conclusions:4.9％of pa-tients with HSIL in cervical cancer screening may be missed or misdiagnosed due to negative HR-HPV typing test.HPV E6/E7 mRNA in situ hybridization detection technology was helpful to reduce the false negative rate of HR-HPV typing test in HSIL,and it may assist in predicting the progression of cervical lesions,so as to provide a new idea for individualized precise treatment.
