PE_PGRS37 protein promotes intracellular colonization of Mycobacterium tuberculosis by inhibiting macrophage autophagy flow
10.3969/j.issn.1002-2694.2025.00.166
- VernacularTitle:PE_PGRS37蛋白通过抑制巨噬细胞自噬流促进结核分枝杆菌的胞内定殖
- Author:
Mengyu LI
1
;
Zhenjun ZHANG
;
Tingting FENG
;
Hui WANG
;
Chanchan NIE
;
Chunwen CHEN
;
Yunjie GAO
;
Yifan DUAN
;
Ruonan GUO
;
Yingying CUI
;
Guanghui DANG
;
Siguo LIU
Author Information
1. 东北农业大学动物医学学院,哈尔滨 150030;中国农业科学院哈尔滨兽医研究所,动物疫病防控全国重点实验室,哈尔滨 150069
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
autophagy;
PE/PPE family protein;
PE_PGRS37
- From:
Chinese Journal of Zoonoses
2025;41(10):1005-1010,1015
- CountryChina
- Language:Chinese
-
Abstract:
This study explored the effect of the Mycobacterium tuberculosis(Mtb)PE/PPE family protein PE_PGRS37 on the growth of Mycobacterium smegmatis(Ms)and macrophage autophagy during Mtb infection.The pe_pgrs37 gene was amplified from Mtb genome through PCR,and the recombinant vector pAIN-PE_PGRS37 was successfully constructed through homologous recombi-nation.pAIN-PE_PGRS37 and pAIN were integrated into Ms through electroshock to construct pAIN-PGRS37/Ms and pAIN/Ms re-combinant bacteria.Western blotting indicated that the PE_PGRS37 protein was correctly expressed in pAIN-PE_PGRS37/Ms.The re-combinant bacteria were inoculated in 7H9/7H10 medium,and their colony morphology and growth curves were observed.No signifi-cant difference in colony morphology was observed between pAIN-PE_PGRS37/Ms and pAIN/Ms.The growth rate significantly in-creased between 10 and 16 h,and a plateau was reached at 26 h.After infection of U937 cells with pAIN-PE_PGRS37/Ms and pAIN/Ms,macrophage autophagy flow was detected with western blotting and immunofluorescence.In the pAIN-PE_PGRS37/Ms-infected group,compared with the pAIN/Ms-infected group,macrophage LC3-II and p62 protein expression was significantly up-regulated(P<0.001)and inhibited autophagosome and lysosome fusion.The intracellular survival of the recombinant bacteria was detected through colony counting,and pAIN-PE_PGRS37/Ms showed significantly greater survival in macrophages at 12 h,24 h,and 48 h than pAIN/Ms(P<0.05).Our results suggested that PE_PGRS37 protein promotes Mycobacterium survival in macrophages by blocking macro-phage autophagy flow,thus inhibiting macrophage autophagy.
