Drug resistance and molecular characteristics of Staphylococcus aureus strains from ICU environment based on whole genome sequencing
10.11816/cn.ni.2025-242087
- VernacularTitle:基于全基因组测序的ICU环境中金黄色葡萄球菌耐药性及其基因特征
- Author:
Yanru LIANG
1
;
Bowen YANG
1
;
Yue ZHANG
1
;
Tongsheng XU
1
;
Bing ZHAO
1
;
Xiao WANG
1
Author Information
1. 上海市浦东新区疾病预防控制中心,上海 200136;复旦大学浦东预防医学研究院,上海 200136
- Publication Type:Journal Article
- Keywords:
Intensive care unit environment;
Staphylococcus aureus;
Drug resistance;
Phylogeny;
Drug resistance gene;
Virulence gene
- From:
Chinese Journal of Nosocomiology
2025;35(14):2170-2175
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the distribution,drug resistance and molecular characteristics of the Staph-ylococcus aureus strains isolated from intensive care unit(ICU)environment in some hospitals of Shanghai Pudong New Area so as to provide bases for prevention and control of infections.METHODS The specimens were collected from ICU environment of 8 medical institutions in Pudong District in 2019 and 2023,the S.aureus strains were i-solated and identified.The drug susceptibility testing was performed by microbroth dilution method.The drug re-sistance genes and virulence genes were predicted based on virulence factors of bacterial pathogens,the compre-hensive antibiotic resistance database(mcmaster.ca)and PubMLST,multilocus sequence typing(MLST)and phylogenetic analysis were performed.RESULTS Totally 3408 samples were collected,and the isolated S.aureus strains accounted for 1.29％(44/3408);the isolated strains form both the shared articles and the articles that health care workers directly contact accounted for 20.45％.The drug resistance rate to penicillin was highest(72.73％,32/44);the strains were completely susceptible to vancomycin,rifampicin,sulfamethoxazole-trime-thoprim,daptomycin and nitrofurantoin.The methicillin-resistant Staphylococcus aureus(MRSA)strains accoun-ted for 25.00％.The multi-drug resistance rate was 40.91％.The strains carried 26 types of drug resistance genes in total,covering 9 categories;the carrying rate of fluroquinolones resistance gene nor A was 100.00％.The strains carried 12 types of enterotoxin genes,the carrying rate of sea gene was highest(50.00％,22/44),some of the strains carried lukS-PV,lukF-PV and tsst gene.There were 14 types of sequence type(ST),dominated by ST7(13/44,29.55％),and novel ST type ST9342 was discovered.The phylogenetic analysis showed that all of the strains were divided into 2 major branches,and the strains with the same ST type were phylogenetically close on the evolutionary tree.CONCLUSIONS The S.aureus strains are prevalent in ICU environment and are highly re-sistant to antibiotics.The strains exhibit various ST types,which,to a certain degree,is associated with the path-ogenicity.
