The effects of short-chain fatty acids on the proliferation,migration and adipogenic ability of dental pulp stem cells
10.3969/j.issn.1001-3733.2025.06.016
- VernacularTitle:短链脂肪酸对牙髓干细胞增殖、迁移及成脂能力影响
- Author:
Yufan WEI
1
;
Xinxia CHANG
;
Xiao ZHANG
;
Genxiong TANG
Author Information
1. 210008,南京医科大学附属儿童医院口腔科
- Publication Type:Journal Article
- Keywords:
Short-chain fatty acids;
Dental pulp stem cells;
Proliferation;
Migration;
Adipogenic ability
- From:
Journal of Practical Stomatology
2025;41(6):816-822
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of short-chain fatty acids(SCFAs)on the proliferation,migration and adipogenic ability of dental pulp stem cells(DPSCs).Methods:Dental pulp stem cells were extracted from young permanent teeth and treated with sodium acetate and sodium propionate.CCK-8 assay was used to detect the activity of DPSCs.Immunofluorescence staining was used to detect the proliferation of DPSCs.The scratch test was employed to assess the migratory activity of DPSCs,alizarin red staining was utilized to evaluate the capacity for calcification nodule formation,and red oil O staining was applied to determine the adipogenic potential of DPSCs.Results:The acetate and propionate significantly enhanced the viability,promoted the proliferation,migration and adipogenic differentiation of DPSCs.The osteogenic differentiation ability of DPSCs,which were with sodium acetate and sodium propionate,was observed.There was no significant difference in the osteogenic differentiation ability of DPSCs compared to the control group.Conclusion:The findings suggest that SCFAs have the potential to enhance the viability,proliferation,migra-tion,and adipogenic differentiation of dental pulp stem cells,thereby promoting their application in pulp repair and tissue engineering.
