Long non-coding RNA HOTTIP promotes the proliferation,migration and invasion of oral cancer cells through MAPK/ERK pathway
10.3969/j.issn.1001-3733.2025.06.009
- VernacularTitle:长链非编码RNA HOTTIP通过MAPK/ERK通路促进口腔癌细胞的增殖、迁移和侵袭
- Author:
Xiaolin SONG
1
;
Qiong HUANG
;
Jing YU
;
Aiying DU
Author Information
1. 810008,西宁市中医院口腔科
- Publication Type:Journal Article
- Keywords:
Oral cancer;
LncRNA HOTTIP;
MAPK/ERK;
Proliferation;
Migration;
Invasion
- From:
Journal of Practical Stomatology
2025;41(6):781-787
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of long non-coding RNA(lncRNA)HOXA transcript at the distal tip(HOT-TIP)in promoting the proliferation,migration and invasion of oral cancer cells via the mitogen activated protein kinase/extracellu-lar regulated protein kinase(MAPK/ERK)pathway.Methods:Human oral cancer KB cells were cultured in vitro and divided into control(without any treatment),pcDNA(transfected with pcDNA-NC,A group),pcDNA-HOTTIP(transfected with pcDNA-HOT-TIP,B group),MAPK/ERK pathway inhibitor PD98059(PD98059 group,50 μmol/L PD98059,C group)and pcDNA-HOTTIP+PD98059 groups(transfected with pcDNA-HOTTIP+50 μmol/L PD98059,D group).qRT-PCR was applied to detect HOTTIP mRNA expression,scratch test was applied to detect KB cell migration,Transwell assay was applied to detect cell invasion,flow cytometry was applied to detect cell apoptosis,CCK-8 was used to detect cell proliferation,Western blot was applied to detect the MAPK/ERK signaling pathway and epithelial mesenchymal transition(EMT)related protein expression levels in KB cells.Results:Compared with the control group,the KB cell scratch healing rate,cell invasion number,A450 value,p-MEK1/2,p-ERK1/2,and N-cadherin expression in pcDNA-HOTTIP group increased,the apoptosis rate and E-cadherin expression reduced(P＜0.05);scratch healing rate,cell invasion number,A450 value,p-MEK1/2,p-ERK1/2,and N-cadherin expression in PD98059 group reduced,the apoptosis rate and E-cadherin expression increased(P＜0.05).PD98059 weakened the role of HOT-TIP in promoting proliferation,migration and invasion of KB cells.Conclusion:LncRNA HOTTIP promotes proliferation,migra-tion,and invasion of KB cells by activating the MAPK/ERK pathway.
