Linc00426 competitively binds to USP3 with miR-455-5p to promote invasion and metastasis of oral squa-mous cell carcinoma cells
10.3969/j.issn.1001-3733.2025.06.008
- VernacularTitle:Linc00426与USP3竞争性结合miR-455-5p促进口腔鳞状细胞癌的侵袭和转移
- Author:
Yanqiao ZHAO
1
;
Lu LIU
;
Xiaona WANG
;
Chonggao YIN
;
Hongli LI
Author Information
1. 261053,潍坊医学院病理学教研室
- Publication Type:Journal Article
- Keywords:
Oral squamous cell carcinoma;
Linc00426;
miR-455-5p;
USP3;
Migration;
Invasion
- From:
Journal of Practical Stomatology
2025;41(6):774-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Linc00426 on the migration,invasion and proliferation of oral squamous carcinoma cells(OSCC).Methods:TCGA database was used to query the differential expression of Linc00426.RT-qPCR was used to detect the expression level of Linc00426 in normal human oral keratinocytes(NHOK)cells and various OSCC cells lines,the knockout effi-ciency of Linc00426 at 3 sites and the expression level of miR-455-5p.Transwell test and EdU test were used to detect the changes of the migration,invasion and proliferation of CAL27 cells.Bioinformatics databases was used to predict subcellular localization and downstream miRNAs and target proteins of Linc00426.Western blot assay was used to detect the changes of USP3 expression levels in CAL27 cells.Results:The expression level of Linc00426 was increased in OSCC tissue(P＜0.05),and its expression in CAL27,HN4 and HN30 cells was higher than that in NHOK cells(P＜0.05).Knocking out Linc00426 inhibited the migration,invasion,and proliferation ability of CAL27 cells(P＜0.05).The bioinformatics database indicates that the subcellular localization of Linc00426 is in the cytoplasm;Linc00426 is negatively correlated with miR-455-5p,and miR-455-5p is negatively correlated with USP3,in which there are targeted binding sites.Knocking out miR-455-5p promoted the migration,invasion and proliferation of CAL27 cells(P＜0.05).Knocking out USP3 inhibited the migration,invasion and proliferation of CAL27 cells(P＜0.05).Knock out Linc00426 reduced the expression level of USP3,while knocking out miR-455-5p increased the expression level of USP3(P＜0.05).Conclusion:Linc00426 plays a role as an oncogene in OSCC cells and could inhibit the migration,invasion and proliferation of the cells by targeting USP3 to regulate miR-455-5p.
