Study on the mechanism of LncRNA DLEU2 affecting the ability of migration and proliferation of oral squamous cell carcinoma cells by regulating the miR-186-5p/IGF2BP3 axis
10.3969/j.issn.1001-3733.2025.06.004
- VernacularTitle:LncRNA DLEU2调控miR-186-5p/IGF2BP3轴影响口腔鳞癌细胞增殖及迁移能力的机制研究
- Author:
Jun MA
1
;
Juan WANG
;
Haiqin WANG
;
Weilin HAN
Author Information
1. 810013,西宁市口腔医院口外颌面外科
- Publication Type:Journal Article
- Keywords:
LncRNA DLEU2;
MiR-186-5p/IGF2BP3 axis;
Oral squamous cell carcinoma;
Proliferation;
Apoptosis;
Migra-tion;
Invasion
- From:
Journal of Practical Stomatology
2025;41(6):750-755
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of LncRNA DLEU2 on the migration and proliferation of oral squamous cell carcino-ma(OSCC)cells by regulating the miR-186-5p/insulin-like growth factor 2 mRNA binding protein 3(IGF2BP3)axis.Methods:QRT-PCR and Western blot were applied to detect the mRNA and protein levels of LncRNA DLEU2,miR-186-5p,and IGF2BP3 in OSCC cell line SCC-25 and human normal oral keratinocyte line NOK,respectively.SCC-25 cells were transfected with si-DLEU2,miR-186-5p inhibitor and negative control respectively.The relationship between LncRNA DLEU2,miR-186-5p and IGF2BP3 was verified by double luciferase reporter gene assay.qRT-PCR was applied to detect the mRNA expression of LncRNA DLEU2 and miR-186-5p in SCC-25 cells.CCK-8 test was applied to detect the proliferation of SCC-25 cells.Flow cytometry was applied to detect the apoptosis rate of SCC-25 cells.Transwell assay was used to detect cell invasion and migration.Western blot was used to detect EMT-associated and IGF2BP3 protein levels.Results:Silencing LncRNA DLEU2 decreased the proliferative ac-tivity,migration and invasion of SCC-25 cells and decreased the protein levels of N-cadherin and Vimentin,increased the apopto-sis rate,miR-186-5p expression and E-cadherin protein levels.Down-regulation of miR-186-5p weakened the inhibitory effect of silenced LncRNA DLEU2 on the malignant phenotype of SCC-25 cells.LncRNA DLEU2 negatively regulated the miR-186-5p/IGF2BP3 axis.Conclusion:Silencing LncRNA DLEU2 may down-regulate the expression of IGF2BP3 by up-regulating miR-186-5p,inhibites the proliferation,migration,and invasion of SCC-25 cells,and promotes the apoptosis of the cells.
