Pathogens and expressions of MEG3/miR-223/NLRP3 axis in peripheral blood for thoracoscopic lobectomy patients with postoperative pulmonary infections
10.11816/cn.ni.2025-241786
- VernacularTitle:胸腔镜肺叶切除术后肺部感染病原菌及外周血MEG3/miR-223/NLRP3轴表达
- Author:
Yaxiong GUO
1
;
Chunhui SHEN
1
;
Ming YANG
1
;
Yanzhong LIU
1
;
Zhenhui WANG
1
;
Guoxiong ZENG
1
;
Beiru WANG
1
;
Jiyong WANG
1
Author Information
1. 广州中医药大学第一附属医院 广东省中医临床研究院胸心外科,广东 广州 510000
- Publication Type:Journal Article
- Keywords:
Lung cancer;
Thoracoscopic lobectomy;
Pulmonary infection;
Pathogen;
Maternal expression gene 3/microribonucleic acid-223/nucleotide-binding oligomerization domain-like receptor protein 3 axis
- From:
Chinese Journal of Nosocomiology
2025;35(14):2110-2114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the distribution of pathogens isolated from the thoracoscopic lobectomy patients with postoperative pulmonary infections and observe the expressions of maternal expression gene 3(MEG3)/microribonucleic acid-223(miR-223)/nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)axis in peripheral blood.METHODS Totally 48 lung cancer patients who underwent thoracoscopic lobec-tomy and had postoperative pulmonary infections in the First Affiliated Hospital of Guangzhou University of Chi-nese Medicine from Jun.2021 to Jun.2024 were assigned as the infection group,meanwhile,31 lung cancer pa-tients who underwent thoracoscopic lobectomy but did not have postoperative infections were chosen as the no in-fection group.The sputum specimens were collected from the infection group,the distribution of isolated patho-gens was analyzed.The clinical data and the expression MEG3,miR-223 and NLRP3 were observed and compared between the two groups.The values of MEG3,miR-223 and NLRP3 in prediction of the postoperative infections were analyzed by receiver operating characteristic(ROC)curves.RESULTS Totally 63 strains of pathogens were i-solated from the 48 thoracoscopic lobectomy patients with postoperative pulmonary infections,33(52.38％)of which were gram-negative bacteria,25(39.68％)were gram-positive bacteria,and 5(7.94％)were fungi.There were significant differences in age,diabetes mellitus,chronic obstructive pulmonary disease(COPD),operation duration,and expression levels of MEG3,miR-223 and NLRP3 between the infection group and the no infection group(P＜0.05).ROC curve analysis showed that the areas under the curves of MEG3,miR-223 and NLRP3 and the joint detection of the three indexes were 0.861,0.760,0.912 and 0.968,respectively,in prediction of the post-operative pulmonary infections,and the predictive efficiency of the joint detection was highest(P＜0.05).CONCLUSIONS The gram-negative bacteria are dominant among the pathogens isolated from the thoracoscopic lo-bectomy patients with postoperative pulmonary infections,the occurrence and progression of the infections may be closely associated with the activation of MEG3/miR-223/NLRP3 axis.The joint detection of the three indexes can effectively predict the postoperative pulmonary infections in the thoracoscopic lobectomy patients.
