Effects of berberine on lipopolysaccharide induced proliferation,apoptosis,inflammation,and osteogenic dif-ferentiation of human periodontal ligament stem cells by regulating the NF-κB/NLRP3 signaling pathway
10.3969/j.issn.1001-3733.2025.06.007
- VernacularTitle:小檗碱调节NF-κB/NLRP3信号通路对脂多糖诱导的人牙周膜干细胞增殖、凋亡、炎症和成骨分化的影响
- Author:
Guangyong HAO
1
;
Xinyu ZHANG
;
Ke LIU
Author Information
1. 453002,新乡市中心医院(东区医院)口腔科
- Publication Type:Journal Article
- Keywords:
Berberine;
Human periodontal ligament stem cells;
Apoptosis;
Osteogenic differentiation;
Proliferation;
Inflammation
- From:
Journal of Practical Stomatology
2025;41(6):768-773
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of berberine on the proliferation,apoptosis,inflammation,and osteogenic differen-tiation of human periodontal ligament stem cells(hPDLSCs)induced by lipopolysaccharide through regulating the NF-κB/NLRP3 signaling pathway.Methods:HPDLSCs with good growth status were separated into a blank group(without any treatment),a lipopolysaccharide group(1 μg/mL lipopolysaccharide,A group),a low concentration berberine(berberine-L)group(1 μg/mL lipopolysaccharide+25 μmol/L berberine,B group),a medium concentration berberine(berberine-M)group(1 μg/mL lipopolysac-charide+50 μmol/L berberine,C group),a high concentration berberine(berberine-H)group(1 μg/mL+100 μmol/L berberine,D group),and a berberine-H+Prostratin group(1 μg/mL lipopolysaccharide+100 μmol/L berberine+1 μmol/L Prostratin,E group).Subsequently,the cell proliferation,levels of interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)in the supernatant,cell apoptosis,osteogenic differentiation ability,expression of osteogenic differentiation related genes[RUNX family transcription fac-tor 2(Runx2),osteopontin(OPN),osteocalcin(OCN)],and expression levels of NF-κB and NLRP3 mRNA were detected.Results:The number of mineralized nodules,cell proliferation rate,ALP content,OPN,OCN,and Runx2 protein expression in the lipopolysac-charide group were greatly reduced compared to the blank group,however,the levels of IL-1 β,IL-6,and TNF-α,apoptosis rate,NF-κB,and NLRP3 mRNA expression in the supernatant were greatly increased(P＜0.05).After treating cells with different doses of berberine,the number of mineralized nodules,proliferation rate,ALP content,OPN,OCN,and Runx2 protein expression were greatly increased,the levels of IL-1β,IL-6,and TNF-α,apoptosis rate,NF-κB,and NLRP3 mRNA expression in the supernatant were greatly reduced,and the berberine-H group was the most great(P＜0.05).After treatment with berberine-H combined with Prostratin,the number of mineralized nodules,proliferation rate,ALP content,OPN,OCN and Runx2 protein expression were greatly reduced compared to single berberine-H treatment,the levels of IL-1β,IL-6 and TNF-α,apoptosis rate,NF-κB,and NLRP3 mRNA expression in the supernatant were greatly increased(P＜0.05).Conclusion:Berberine enhances lipopolysaccharide induced proliferation of hPDLSCs,inhibits apoptosis and inflammation,and promotes osteogenic differentiation by inhibiting the NF-κB/NLRP3 signaling pathway.
