Determination of tulobuterol in dried urine spot and dried blood spot in the presence of bupropion using LC-HRMS
10.3969/j.issn.1000-6710.2025.10.006
- VernacularTitle:使用LC-HRMS法检测安非他酮存在下干尿点和干血点中的妥洛特罗
- Author:
Junxiu LIN
1
;
Xinyuan REN
;
Jing JING
Author Information
1. 上海体育大学运动健康学院(上海 200438)
- Publication Type:Journal Article
- Keywords:
LC-HRMS;
dried blood spot;
dried urine spot;
tulobuterol;
bupropion
- From:
Chinese Journal of Sports Medicine
2025;44(10):821-832
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a qualitative detection method for tulobuterol in dried urine spots(DUS)as derivatives produced after administration of the non-prohibited substance bupropion can inter-fere with the detection of the doping tulobuterol in urine,and further explore the interference in blood samples and find a method for its detection in dried blood spots(DBS).Methods Ten volunteers(5 males and 5 females)were chosen for method validation,and another 2 volunteers(1 male and 1 fe-male)were recruited for the bupropion study.Totally 20 μL samples of DUS and DBS were collected separately.After pretreatment steps such as liquid-liquid extraction with mixed organic solvents and ni-trogen blowing for re-dissolution,the detection and analysis were carried out using liquid chromatogra-phy-high resolution mass spectrometry(LC-HRMS).Results The limits of detection(LOD)for tulobuter-ol in both DUS and DBS samples were 0.13 ng/mL,respectively,while the limit of identification(LOI)were 0.16 ng/mL and 0.90 ng/mL,respectively.Meanwhile,the repeatability and reproducibility relative standard deviation(RSD%)were<20%for different concentrations,with recovery rate of 38.65%～70.01%and the matrix effect of 37.98%～142.13%,and the stability of the samples could reach 30 days in different storage conditions.As to the bupropion study,its derivatives could be effectively sepa-rated from tulobuterol in DUS samples,but were not detected in DBS samples,which directly ex-cludes their interference.Conclusion Detection of tulobuterol in DUS and DBS using LC-HRMS can eliminate interference from bupropion derivatives in the detection of the doping tulobuterol.
