The Role of Platelet-Derived Zyxin in Promoting Tumor Migration
10.19746/j.cnki.issn1009-2137.2025.06.023
- VernacularTitle:血小板源zyxin在促进肿瘤迁移中的作用
- Author:
Meng-Nan YANG
1
;
Shuang CHEN
1
;
Li-Li ZHAO
1
;
Kang-Xi ZHOU
1
;
Rong YAN
1
;
Ke-Sheng DAI
1
;
Xin-Xin GE
1
Author Information
1. 苏州大学附属第一医院血液科,江苏省血液研究所,卫健委血栓与止血重点实验室,放射医学与防护国家重点实验室,血液学协同创新中心,江苏苏州 215006
- Publication Type:Journal Article
- Keywords:
platelet;
zyxin;
skeleton protein;
tumor metastasis
- From:
Journal of Experimental Hematology
2025;33(6):1708-1713
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of platelet-derived zyxin in promoting tumor migration by platelets.Methods:The gene expression profile of platelets was analyzed from cancer patients by using the GEO database.Isolated platelets from wild-type(WT)and Zyx-/-mice were co-cultured with B16F10 cells labeled with green fluorescence to investigate the influence of zyxin deficiency on tumor cell migration,invasion,and wound healing.Optical microscopy was employed to evaluate the impact of zyxin deficiency on epithelial-mesenchymal transition(EMT)in B16F10 cells induced by platelets.Employing specific markers to label platelets,fluorescence confocal microscopy was utilized to investigate the impact of platelet-derived zyxin on the binding between tumor cells and platelets.And an aggregometer was employed to observe the influence of zyxin deficiency on tumor cell-induced platelet aggregation.Results:Compared to platelets from healthy volunteers,zyxin was upregulated in platelets from cancer patients.Zyx-/-mouse platelets exhibited a significant reduction in tumor cell invasion and migration,impaired wound healing,and delayed tumor cell EMT compared to WT mouse platelets.Additionally,zyxin deficiency attenuated the interaction between platelets and tumor cells,and diminished the capacity for tumor cell-induced platelet aggregation.Conclusion:Platelet-derived zyxin deficiency diminishes platelet-tumor cell interactions and weakens the ability of tumor cell-induced platelet aggregation,ultimately suppressing tumor cell migration.
