Drug resistance genes and efflux pump phenotypes in clinically isolated Acinetobacter baumannii
10.11816/cn.ni.2025-241402
- VernacularTitle:临床分离鲍曼不动杆菌耐药基因及外排泵表型分布
- Author:
Li LI
1
;
Zhizhen ZHANG
1
;
Xiaoling ZOU
1
;
Xu WANG
1
;
Jiao DAI
1
Author Information
1. 都江堰市人民医院呼吸与危重症医学科,四川都江堰 611830
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Carbapenem;
β-lactamase gene;
Efflux pump system;
Distribution
- From:
Chinese Journal of Nosocomiology
2025;35(11):1707-1710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the epidemiological characteristics of Acinetobacter baumannii,detect the distribution of its β-lactamase genes and efflux pump genes,and analyze the relationship between β-lactamase genes and efflux pump genes and the drug resistance of the strains.METHODS Acinetobacter baumannii strains clinically isolated from Jun.2021 to Jun.2022 in Dujiangyan People's Hospital were collected and tested for anti-microbial resistance.Polymerase chain reaction(PCR)technology was used to amplify the β-lactamase genes as well as the efflux pump genes of Acinetobacter baumannii.PaβN was performed to test the efflux pump pheno-type.RESULTS The drug resistance rates of imipenem-resistant Acinetobacter baumannii(IRAB)to cefopera-zone,ceftazidime,cefepime,imipenem,and meropenem were 14.29％,85.71％,91.43％,100.00％and 100.00％,respectively,significantly higher than those of imipenem-sensitive Acinetobacter baumannii(ISAB)(P＜0.05).The detection rates of OXA-23,OXA-51,and IMP-1 in IRAB strains were 45.71％,62.86％and 65.71％,respectively,higher than those in ISAB strains(P＜0.05).The detection rates of the efflux pump genes adeB,adeS,and adeG in IRAB strains were 60.00％,71.43％and 68.57％,respectively,higher than those in ISAB strains(P＜0.05).In addition,the positive rate of the efflux pump phenotype in the IRAB group was 54.29％,significantly higher than 7.69％in the ISAB group(P＜0.001).CONCLUSION The detection rates of β-lactamase genes(such as OXA-23,OXA-51 and IMP-1 etc.)and efflux pump genes(adeB,adeS and adeG)in IRAB are significantly higher than those in the ISAB,and the positive rate of the efflux pump phenotype in IRAB is higher,which confirms that β-lactamase genes and efflux pump genes are important mechanisms of drug resistance in the bacterium.
