Efficacy of low temperature plasma in treatment of rats with Pseudomonas aeruginosa wound infection
10.11816/cn.ni.2025-241075
- VernacularTitle:低温等离子体对大鼠铜绿假单胞菌感染创面的修复作用
- Author:
Jiali ZHANG
1
;
Ming ZHOU
;
Gongzi ZHANG
;
Yangxiaoxue LIU
;
Tianshu ZHAO
;
Min XIN
;
Gang WANG
;
Liping HUANG
Author Information
1. 解放军总医院第一医学中心康复医学科,北京 100853
- Publication Type:Journal Article
- Keywords:
Low temperature plasma;
Pseudomonas aeruginosa;
Wound infection;
Requirement for healing;
Bac-terial load
- From:
Chinese Journal of Nosocomiology
2025;35(13):1921-1926
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the efficacy of low temperature plasma(LTP)in treatment of the rats with Pseudomonas aeruginosa(PA)wound infection.METHODS Totally 20 SD rats were assigned to establish the PA wound infection models and were randomly divided into the LTP group with 10 rats and the air control group with 10 rats.The wound healing dynamics,bacterial loading capacity,histopathology,levels of cytokines and microen-vironment features were observed during the healing process.RESULTS LTP accelerates the healing of PA infec-tion wound,the wound area reduced after the treatment for 3 days,and the inflammatory reactions alleviated;the infection symptoms subsided after the treatment for 7 days,and the wounds became dry and scabbed;the wounds reduced in size by day 15,showing favorable healing trends.Repeated measurement analysis of variance(ANOVA)indicated that there was significant difference in the impact of LTP on the covering rate of wounds(Ftime=10.230,P＜0.001;Fbetween-group=11.340,P=0.001;Finteraction=2.890,P=0.042).The result of quantitative bacterial loads analysis showed that the bacterial loads of the LTP group were lower than those of the control group after the treatment for 3,7 and 15 days(P＜0.05).The result of histopathology indicated that the infiltra-ted wound inflammatory cells of the LTP group reduced,the collagen fibers were well-aligned,and the neovascu-larization and formation of granulation tissues were evident.In addition,the pH value of wound tissues and reac-tive oxygen species(ROS)level were improved after the treatment with LTP,which was matched with the process of wound healing.The result of cytokines test showed that LTP could upregulate the levels of VEGF and TGF-β1,downregulate the levels of IL-1β and TNF-α,and promote the wound healing,with statistically signifi-cant differences observed(P＜0.05).CONCLUSION LTP may facilitate the control of infections by reducing the bacterial colony counts,establish the effective microenvironment for tissue healing,and accelerate the healing of wounds with PA infection by regulating the release of healing-related cytokines.
