Effects of nobiletin on proliferation and apoptosis of laryngeal squamous cell carcinoma cells by regulating the FAK/AKT signaling pathway
- VernacularTitle:川陈皮素调节FAK/AKT信号通路对喉鳞状细胞癌细胞增殖和凋亡的影响
- Author:
Jiancun HAN
1
;
Yi ZHOU
1
Author Information
- Publication Type:Journal Article
- Keywords: laryngeal neoplasms; carcinoma,squamous cell; nobiletin; focal adhesion protein-tyrosine kinases; proto-oncogene proteins c-akt; cell proliferation; apoptosis
- From: Tianjin Medical Journal 2025;53(6):561-565
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of nobiletin on the proliferation and apoptosis of laryngeal squamous cell carcinoma(LSCC)cells and its mechanism related to the focal adhesion kinase(FAK)/protein kinase B(AKT)signaling pathway.Methods TU177 cells of LSCC were randomly assigned into the control group,the L-nobiletin group(10 mg/L)group,the M-nobiletin group(20 mg/L),the H-nobiletin group(40 mg/L)and the H-nobiletin+FAK activator ZINC40099027 group(40 mg/L nobiletin+2.5 g/L ZINC40099027).CCK-8 method was used to detect cell viability.Flow cytometry was used to detect cell apoptosis.Scratch experiment was used to detect cell migration.Transwell method was used to detect cell invasion.Western blot assay was used to detect apoptosis and FAK/AKT pathway related proteins.Results Compared with the control group,the cell viability,scratch healing rate,number of invasive cells,the protein expressions of Bcl-2,p-FAK/FAK and p-AKT/AKT were decreased in a dose-dependent manner in the L-nobiletin group,the M-nobiletin group and the H-nobiletin group,while the cell apoptosis rate and protein expressions of Bax and caspase-3 were increased in a dose-dependent manner(P＜0.05).The OD value,scratch healing rate,number of invasive cells,Bcl-2,p-FAK/FAK and p-AKT/AKT protein expression levels were higher in the H-nobiletin+ZINC40099027 group than those of the H-nobiletin group,and cell apoptosis rate,Bax and caspase-3 protein expression were lower compared to the H-nobiletin group(P＜0.05).Conclusion Nobiletin may inhibit the proliferation and promote the apoptosis of LSCC cells by suppressing the FAK/AKT signaling pathway.
