Analysis of Chemical Constituents and Blood-Absorbed Ingredients of Heihuang Chizhu Granules by UPLC-Q-TOF-MS/MS
10.14148/j.issn.1672-0482.2025.0749
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术的黑黄赤珠颗粒化学成分及入血成分分析
- Author:
Yiwei HUANG
1
;
Lijiao HUANG
;
Junhua HU
;
Zhenzhong WANG
;
Chenfeng ZHANG
;
Zhongkun XU
;
Yat-ing LI
;
Juan FU
;
Wei XIAO
Author Information
1. 中药制药过程控制与智能制造技术全国重点实验室(江苏康缘药业股份有限公司/南京中医药大学),江苏 南京 210000;南京中医药大学康缘中药学院,江苏 南京 210023
- Publication Type:Journal Article
- Keywords:
Heihuang Chizhu Granules;
chemical constituents;
constituents absorbed into blood;
UPLC-Q-TOF-MS/MS;
compo-sitional analysis
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(6):749-765
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the chemical constituents of Heihuang Chizhu Granules and the blood composition of rats af-ter administration by UPLC-Q-TOF-MS/MS.METHODS A Waters ACQUITY UPLC HSS T3 column(3 mm×100 mm,1.8 μm)was eluted with acetonitrile-0.1%formic acid as mobile phase,and the data were collected in electrospray ion source positive and neg-ative ion mode and then identified with the reference retention time,precise molecular weight,secondary fragment ions,and references to relevant literature.RESULTS A total of 104 chemical components were identified from Heihuang Chizhu Granules,including 26 flavonoids,24 organic acids,14 triterpenoids,8 terpenoids,7 phenylpropanoids,11 monoglycosides,and 14 other components(phe-nols,alkaloids,etc.).On this basis,39 blood-entering components were identified in the plasma of rats administered via gavage,in-cluding 28 prototypes and 11 metabolites.CONCLUSION The chemical constituents of Heihuang Chizhu Granules and the compo-nents entering the blood of rats are analyzed and identified for the first time in this study,and the results provide a scientific basis for the basic research of Heihuang Chizhu Granules and the establishment of process quality control standards.
