Effect and mechanism of BCG immunotherapy in mice melanoma model
10.3969/j.issn.1000-484X.2025.06.027
- VernacularTitle:卡介苗用于小鼠黑色素瘤模型免疫治疗的效应及其机制
- Author:
Mingze XU
1
;
Huanhuan NING
;
Yanzhi LU
;
Jian KANG
;
Yujun PENG
;
Jingyao ZHANG
;
Jiahao HU
;
Ting DAI
;
Mengjuan DONG
;
Sa XUE
;
Yinlan BAI
Author Information
1. 空军军医大学基础医学院微生物与病原生物学教研室,西安 710032
- Publication Type:Journal Article
- Keywords:
BCG;
Recombinant BCG;
Melanoma;
c-di-AMP;
Immunotherapy
- From:
Chinese Journal of Immunology
2025;41(6):1420-1426
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate immunotherapy effects and mechanism of BCG and recombinant BCG(rBCG)with c-di-AMP as adjuvant on melanoma in mice model.Methods:Melanoma mice model was established by B16F10 cell subcutaneous injec-tion in groin,and treated with 1×106 CFU of BCG and rBCG by adjacent injection of subcutaneous tumor for 3 times,respectively.Survival of melanotic mice,tumor growth and metastasis were observed.Tumor tissues of mice were isolated to prepare cell suspen-sion,and proportion of immune cells were detected by flow cytometry.Transcriptional levels of immune-related genes in tumor tissues were detected by qRT-PCR.Results:Both BCG and rBCG immunotherapy could significantly inhibit growth in melanoma mice and prolong survival time of mice.rBCG showed better inhibition on metastasis than BCG.Both strains significantly reduced proportion of M2-type macrophages and myeloid-derived suppressor cell associated with tumor growth and metastasis.Both two strains promoted infiltration of lymphocytes in tumor tissues,and rBCG significantly increased proportion of B cells in tumor.BCG immunotherapy upregulated transcription levels of metastasis-related cytokines,while rBCG therapy had no effects on transcriptions of these genes.Conclusion:Both BCG and rBCG have immunotherapeutic effects on melanotic mice,and rBCG with c-di-AMP as adjuvant shows better inhibition on tumor metastasis than BCG,which mechanism was related to regulation of immune response in tumor tissues.
