Effect of extracellular matrix extract of decellularized extracellular matrix material on immune characteristics of RAW264.7 macrophages
10.3969/j.issn.1000-484X.2025.06.017
- VernacularTitle:脱细胞化细胞外基质材料浸提液对RAW264.7巨噬细胞免疫特性的影响
- Author:
Juwen MA
1
;
Hang ZHANG
;
Yunwen WANG
;
Yongwei WANG
;
Hongru SONG
;
Yaxian GAO
Author Information
1. 承德医学院基础医学院免疫学教研室,承德 067000
- Publication Type:Journal Article
- Keywords:
dECM;
Decellularized extracellular matrix;
Macrophage polarization;
ROS;
Inflammation
- From:
Chinese Journal of Immunology
2025;41(6):1358-1364
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of decellularised extracellular matrix material(dECM)extract on macrophage survival,migration,phagocytosis,pro-inflammatory factor expression and ROS production.Methods:CCK-8 method was used to detect whether the extract of dECM material had cytotoxicity on macrophages;effects of dECM material extracts on macrophage recruit-ment and chemotaxis were examined by Transwell migration assay;effects of dECM material extract on macrophage phagocytosis were detected by pHrodo staining;effects of dECM material extract on expression of pro-inflammatory genes and pro-inflammatory specific functional molecules were detected by RT-qPCR and immunofluorescence staining;detection of the effect of dECM material extracts on macrophage ROS production by the DCFH-DA fluorescent probe approach.Results:Compared with DMEM complete medium,①CCK-8 assay showed that dECM material extract had no toxic effect on macrophages,and could promote the formation of macrophage colonies;②pHrodo staining assay showed that dECM material extract did not affect phagocytosis of macrophages;③Transwell migra-tion assay showed that dECM material extract did not promote macrophage migration;④RT-qPCR results showed that dECM material extracts were able to down-regulate gene expressions of pro-inflammatory cytokines and specific functional molecules;⑤Flow cytome-try results showed that dECM material extract could reduce the production of ROS by macrophages.dECM material extract had excel-lent biocompatibility for macrophages.Conclusion:dECM material extract is non-toxic to macrophages and is able to reduce macro-phage pro-inflammatory gene expression and ROS production.
