Glutamate Acid Chemical Exchange Saturation Transfer Imaging in Hippocampus of Depressed Rats Based on 7T Magnetic Resonance Imaging
10.3969/j.issn.1005-5185.2025.05.010
- VernacularTitle:基于7T磁共振成像的抑郁症大鼠海马谷氨酸化学交换饱和转移成像研究
- Author:
Linxuan ZHANG
1
;
Songtao WANG
1
;
Kai QI
1
;
Chang XU
1
;
Yingxin LIU
1
;
Wei ZHANG
1
Author Information
1. 滨州医学院医学影像学院,山东 烟台 264003
- Publication Type:Journal Article
- Keywords:
Depression;
Magnetic resonance imaging;
Chemical exchange saturation transfer;
Glutamic acid;
Ketamine;
Rats,Sprague-Dawley
- From:
Chinese Journal of Medical Imaging
2025;33(5):507-511
- CountryChina
- Language:Chinese
-
Abstract:
Purpose Chemical exchange saturation transfer(CEST)imaging is used to study the changes of glutamate metabolism in the hippocampus of rats with chronic unpredictable mild stress(CUMS)model,so as to evaluate the clinical reference value of glutamate acid CEST(GluCEST)imaging results.Materials and Methods Twenty-two male SD rats were enrolled,and were divided into CUMS and healthy groups.Rats in CUMS group were further divided into the non-treatment group(n=7)and the ketamine treatment group(n=8).Seven healthy rats were randomly selected as control group.CEST imaging scans were performed using 7T small animal magnetic resonance and glutamate concentrations were measured in both hippocampi.The difference of hippocampal GluCEST value and glutamate concentration between control group and CUMS non-treatment group,CUMS ketamine treatment group and CUMS non-treatment group was analyzed,respectively.Results Compared with the control group,the hippocampal GluCEST value in CUMS non-treatment group was increased(left:t=2.8,P=0.015;right:t=3.0,P=0.011),while the hippocampal GluCEST value of rats in CUMS ketamine treatment group was decreased compared with CUMS non-treatment group(left:t=2.3,P=0.037;right:t=2.5,P=0.028).Conclusion GluCEST imaging can provide high spatial resolution images and accurately evaluate the changes of glutamate metabolism in hippocampus of rats with depression,which is conducive to monitoring the abnormal signals of hippocampal neurons caused by depression.
