Effects of moxibustion at Tianshu(ST25)and Qihai(CV6)on ACE/Ang Ⅱ/AT1R axis in the colon tissue of Crohn disease mice with intestinal fibrosis
10.1007/s11726-025-1502-4
- VernacularTitle:艾灸天枢和气海对克罗恩病肠纤维化小鼠结肠ACE/Ang Ⅱ/AT1R轴的影响
- Author:
Yang PENG
;
Qianqian GAO
;
Qianru FENG
;
Xiaoji ZHU
;
Zhe MA
;
Huangan WU
;
Huirong LIU
;
Cili ZHOU
- Publication Type:Journal Article
- Keywords:
Moxa Stick Moxibustion;
Point,Tianshu(ST25);
Point,Qihai(CV6);
Crohn Disease;
Angiotensin-converting Enzyme;
Angiotensin Ⅱ;
Angiotensin Ⅱ Type 1 Receptor;
Mice
- From:
Journal of Acupuncture and Tuina Science
2025;23(3):197-209
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the therapeutic mechanism of moxibustion in Crohn disease(CD)-associated intestinal fibrosis by observing its effects on the angiotensin-converting enzyme(ACE)/angiotensin Ⅱ(Ang Ⅱ)/angiotensin Ⅱ type 1 receptor(AT1R)axis in CD mouse models.Methods:Six randomly selected male C57BL/6 mice were assigned to a normal group,while the remaining mice were administered 0.1 mL of 2,4,6-trinitrobenzene sulfonic acid via enema to establish a CD intestinal fibrosis model.After successful modeling,the mice were randomly divided into a model group,a moxibustion group,and a Western medication group,with 6 rats in each group.The normal group and the model group only received grabbing without intervention.In the moxibustion group,mild moxibustion was applied to Qihai(CV6)and bilateral Tianshu(ST25)once a day for 10 min each time over 7 consecutive days.The Western medication group was administered mesalazine suspension via oral gavage once a day for 7 consecutive days.At the end of the intervention,the general condition,disease activity index(DAI)score,and gross colon score of mice in each group were evaluated.Hematoxylin-eosin staining was used to observe and score the histological changes in the colon tissue in each group.Masson staining was used to observe colonic fibrosis and the ratio of collagen-positive areas was analyzed;the expression of Ang Ⅱ in the colon tissue was detected by the enzyme-linked immunosorbent assay;immunohistochemistry and real-time quantitative reverse transcription polymerase chain reaction were used to detect the protein and mRNA expression of ACE and AT1R in the colon tissue,respectively;Western blotting was used to detect the expression of transforming growth factor(TGF)-β1 and connective tissue growth factor(CTGF)in the colon tissue.Results:Compared to the normal group,the DAI score,gross colon score,colonic histological score,collagen-positive area ratio,ACE protein and mRNA,Ang Ⅱ protein,AT1R protein and mRNA,TGF-β1 protein,and CTGF protein in the colon tissue in the model group increased significantly(P<0.01).In contrast,the above indicators in both the moxibustion group and the Western medication group reduced significantly compared to the model group(P<0.01 or P<0.05).There was no statistical difference in these indicators between the moxibustion group and the Western medication group(P>0.05).Conclusion:Moxibustion can alleviate intestinal fibrosis in CD mice,and its therapeutic mechanism may be associated with the regulation of colonic ACE/AngⅡ/AT1R axis.
