Association of expressions of TGF-β1,Smad2 and Smad3 with pulmonary infection in patients with primary nephrotic syndrome
10.11816/cn.ni.2025-241208
- VernacularTitle:TGF-β1、Smad2、Smad3表达与原发性肾病综合征合并肺部感染的关系
- Author:
Huicong LI
1
;
Xiaoguang ZHU
1
;
Jun SHI
1
Author Information
1. 河南大学淮河医院肾内科,河南开封 475000
- Publication Type:Journal Article
- Keywords:
Primary nephrotic syndrome;
Pulmonary infection;
Transforming growth factor-β1/Smads signaling pathways;
Illness condition
- From:
Chinese Journal of Nosocomiology
2025;35(9):1311-1316
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the expressions of transforming growth factor-β1(TGF-β1)/Smads signaling pathways in the primary nephrotic syndrome(NS)patients complicated with pulmonary infection and analyze the values in diagnosis of pulmonary infection and assessment of illness condition.METHODS A total of 108 NS pa-tients complicated with pulmonary infection who were treated in Huaihe Hospital of Henan University from Jan.2020 to Mar.2024 were assigned as the infection group and were divided into the mild group with 32 cases and the severe group with 76 cases according to the severity of pulmonary infection.The distribution of the pathogens was analyzed.Meanwhile,97 NS patients who were not complicated with pulmonary infection were chosen as the no infection group.The relative expression levels of peripheral blood TGF-β1,Smad2,Smad3 mRNA and proteins were detected for the patients confirmed with infection within 24 hours(for the patients with infection within 24 hours after the admission)by means of real-time fluorescent quantitative polymerase chain reaction.The values of the three indexes in diagnosis of the pulmonary infection and assessment of illness condition of the NS patients were analyzed.RESULTS Totally 122 strains of pathogens were isolated from sputum specimens of the 108 NS pa-tients complicated with pulmonary infection,78.69％of which were gram-negative bacteria.The relative expres-sion levels of TGF-β1,Smad2,Smad3 mRNA and proteins of the infection group were respectively 8.69±1.33,6.07±0.75 and 5.94±0.88,higher than 3.76±0.3,2.38±0.24 and 3.15±0.35 of the no infection group(P＜0.05);the levels of the indexes of the severe group were higher than those of the mild group(P＜0.05).Receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC)of the joint detection of TGF-β1,Smad2 and Smad3 was 0.972 in diagnosis of the pulmonary infection in the NS patients,0.912 in as-sessment of the severe pulmonary infection.CONCLUSIONS The NS patients complicated with pulmonary infection show remarkable rise of expressions of TGF-β1,Smad2 and Smad3.The joint detection of the above indexes has high clinical value in diagnosis of the pulmonary infection and assessment of illness condition.
