Study on the HPLC Characteristic Chromatogram and Quantitative and Qualitative Transfer of Five Index Components in Tra-ditional Chinese Formula Yunvjian
10.16466/j.issn1005-5509.2025.05.001
- VernacularTitle:经典名方玉女煎HPLC特征图谱及5种指标成分的量质传递研究
- Author:
Chengyan LI
1
;
Qitao YE
;
Yun ZHOU
Author Information
1. 浙江中医药大学药学院 杭州 311400
- Publication Type:Journal Article
- Keywords:
traditional Chinese formula;
Yunvjian;
references sample;
characteristic chromatogram;
content determination;
high performance liquid chromatography;
quantitative and qualitative transfer;
quality control
- From:
Journal of Zhejiang Chinese Medical University
2025;49(5):525-535
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To establish the high performance liquid chromatography(HPLC)characteristic chromatogram and multi-index contents determination method of Yunvjian,clarify the quantitative and qualitative transfer law from decoction pieces-references sample-granules.[Methods]The HPLC characteristic chromatogram of Yunvjian references sample and granules was established using Thermoscientific Syncronis aQ C18 chromatographic column(150 mm×4.6 mm,5 μm),acetonitrile(A)-0.1%phosphoric acid water(B)as mobile phase,detection wavelength as 240 nm,adopting gradient elution.Five index components,including rehmannioside D,neomangiferin,mangiferin,β-ecdysterone and acteoside,were selected to establish an HPLC method with detection wavelengths of 203 nm and 240 nm.The content of the index components and the transfer rate in different samples were calculated.[Results]The similarity of the HPLC characteristic chromatograms of 10 batches of the Yunvjian references samples and 3 batches of granules is all greater than 0.950.A total of 12 common peaks are attributed,and 6 of them are identified.All the 12 common peaks in the reference samples are stably transferred to the granules.The 5 index components showed excellent linearity(r≥0.9999)within their respective concentration ranges,and the average sample recovery rate is 99.16%～102.26%.The methodological investigation of the content determination meets the relevant requirements.During the process of preparing the Yunvjian from decoction pieces to granules,the transfer rates of the 5 components are 46.31%～52.30%,31.89%～41.46%,28.65%～33.85%,41.28%～48.59%and 49.59%～75.20%,respectively,all within the range of the average value±30%.[Conclusion]The HPLC characteristic chromatogram and content determination method established are accurate and reliable,and the quantitative and qualitative transfer law of decoction pieces-granules is relatively stable,which can provide an objective basis for the preparation development and quality control of Yunvjian.
