Preparation,drug loading,and immune activation of genetically engineered bacterial vesicles
10.3969/j.issn.1005-202X.2025.06.018
- VernacularTitle:基因工程化细菌囊泡的制备、载药与免疫激活
- Author:
Yuji TANG
1
;
Jiazhen TANG
;
Lang RAO
;
Yahong WEI
Author Information
1. 西北农林科技大学生命科学学院,陕西 杨凌 712100;深圳湾实验室化学生物学研究所,广东 深圳 518132
- Publication Type:Journal Article
- Keywords:
gene modification;
interferon α;
bacterial vesicle;
drug delivery;
photodynamic therapy
- From:
Chinese Journal of Medical Physics
2025;42(6):825-831
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor effect of genetically engineered bacterial vesicles loaded with the photosensitizer Ce6.Methods Genetic engineering techniques were used to overexpress interferon α(IFNα)in Escherichia coli Nissle 1917(EcN),and the corresponding bacterial vesicles(BVs-IFNα)were extracted.The photosensitizer Ce6 was loaded onto the surface of the bacterial vesicles via ultrasonication to obtain BVs-IFNα@Ce6 nanoparticles.The BVs-IFNα@Ce6 complex was characterized using dynamic light scattering,nanoparticle tracking technology,confocal imaging,and ultraviolet absorption spectroscopy.The obtained BVs-IFNα@Ce6 nanoparticles were co-cultured with mouse melanoma cells(B16F10)and mouse bone marrow-derived dendritic cells(BMDC).The efficacy of photodynamic therapy was assessed using the CCK-8 method,and its ability to induce immune activation was verified using flow cytometry.Results The BVs-IFNα@Ce6 nanoparticles were successfully prepared with a stable particle size of around 180 nm.Under red light irradiation,BVs-IFNα@Ce6 exhibited significant cytotoxicity against B16F10 tumor cells and effectively promoted the expressions of immune-related molecules MHC-II,CD80/CD86 on the surface of BMDC cells,demonstrating its remarkable ability to induce immune activation.Conclusion The BVs-IFNα@Ce6 nanoparticles possess dual functions of enhancing photodynamic therapy efficacy and activating antitumor immunity,showing potential for clinical translation.
