Effects of Jiaotai Pills on high-fat diet-induced hypothalamic inflammation in obese mice
10.3969/j.issn.1001-1528.2025.02.013
- VernacularTitle:交泰丸对高脂饮食诱导肥胖小鼠下丘脑炎症的改善作用
- Author:
Hui WANG
1
;
Lin YUAN
1
;
Na HU
1
;
Min LIN
1
;
Yi JIANG
1
;
Min LU
1
;
Xiao-nan WANG
1
;
Xiong LU
1
;
Xiao-yu ZHONG
1
Author Information
1. 上海中医药大学科技实验中心细胞生物与组织病理学实验室,上海 201203
- Publication Type:Journal Article
- Keywords:
Jiaotai Pills;
hypothalamus;
inflammation;
glial cells;
TLR4/NF-κB signaling pathway
- From:
Chinese Traditional Patent Medicine
2025;47(2):446-452
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of Jiaotai Pills and their single composition drugs on high-fat diet-induced hypothalamic inflammation in obese mice.METHODS C57BL/6J mice were randomly divided into the normal group(15 mice)and the high-fat group(75 mice).The mice given 12 weeks of high-fat diet feeding were further randomly divided into the model group,the Jiaotai Pills group,the Coptis chinensis group,the Cinnamomum cassia group and the positive metformin group,with 15 mice in each group.After 6 weeks of administration,the mice had their body weight and fasting blood glucose(FBG)levels detected;their hypothalamic expressions of IL-1β,IL-6,TNF-α and Socs3 mRNA detected by RT-qPCR;their hypothalamic expressions of TLR4,MyD88,IKKβ and activated NF-κB protein detected by Western blot;their hypothalamic expressions of Iba1 and GFAP detected by immunohistochemistry;and their ultrastructural changes of nerve tissues observed using transmission electron microscopy(TEM).RESULTS Compared with the model group,each drug group displayed decreased hypothalamic expressions of IL-1β,IL-6,TNF-α and Socs3 mRNA(P＜0.01),and improved number and morphology of nerve cells revealed by TEM.The groups intervened with Jiaotai Pills,or Coptis chinensis,or metformin shared decreased body weight and FBG levels(P＜0.05);decreased protein expressions of TLR4,MyD88,IKKβ and p-NF-κB(P＜0.05);and decreased number of hypothalamic astrocytes and microglia(P＜0.05).Additionally,decreased p-NF-κB protein expression was observed in the Cinnamomum cassia group(P＜0.05).CONCLUSION Jiaotai Pills and their single composition drugs can improve high-fat diet-induced hypothalamic inflammation in obese mice.
