Comparison of six active constituent contents in modified Liujunzi Decoction during different process amplifications
10.3969/j.issn.1001-1528.2025.02.006
- VernacularTitle:加味六君子合剂在不同工艺放大过程中6种活性成分含量比较
- Author:
Ya-ping ZHU
1
;
Yu-xin LIU
;
Meng-qi SHAO
;
You-jin WANG
;
Lei WU
Author Information
1. 南京中医药大学附属医院,江苏南京 210029;南京中医药大学第一临床医学院,江苏南京 210029
- Publication Type:Journal Article
- Keywords:
modified Liujunzi Decoction;
active constituents;
small test;
pilot test;
large production;
HPLC fingerprints;
content determination
- From:
Chinese Traditional Patent Medicine
2025;47(2):395-400
- CountryChina
- Language:Chinese
-
Abstract:
AIM To compare the contents of caffeic acid,ferulic acid,narirutin,calycosin,glycyrrhizic acid and atractylenolide Ⅲ of modified Liujunzi Decoction(MLJZD)during small test,pilot test(500,1 500 L)and large production.METHODS The samples were taken after soaking for 60 min,boiling for 0,5,10,15,20,30 min in the first decoction,and boiling for 5,10,15,20 min in the second decoction,respectively,after which the HPLC fingerprints were established,the contents of active constituents were determined.RESULTS There were 6 common peaks in the HPLC fingerprints for small test and pilot test,while 5 common peaks were observable in the HPLC fingerprints for large production,along with the similarities of more than 0.980.During pilot tests at different time points,various active constituents demonstrated consistent content changing trends,whose total content was higher than those during small test and large production.CONCLUSION Process amplification exhibits a little influence on active constituent contents in MLJZD,which don't show increasing trends with the expansion of container and enhancement of dosage.
