Expression and validation of the neurotrophin neuritin in human tissues
10.12007/j.issn.0258-4646.2025.06.005
- VernacularTitle:神经营养因子neuritin在人体组织中的表达及验证
- Author:
Pingping MENG
1
;
Jingling ZHU
;
Jian CAO
;
Yu WEI
;
Xing LUO
Author Information
1. 石河子大学医学院医学遗传学教研室,新疆石河子 832002
- Publication Type:Journal Article
- Keywords:
neuritin;
tissue distribution;
expression profile;
immunohistochemistry;
subcellular localization
- From:
Journal of China Medical University
2025;54(6):505-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression and validate the localization of the neurotrophin neuritin(NRN1)in human tissues using bioinformatics and experimental methods.Methods The tissue-specific expression of NRN1 was analyzed using the Human Pro-tein Atlas(HPA)database.NRN1 mRNA and protein expression were experimentally validated using real-time quantitative PCR and immunohistochemistry,respectively.A pEGFP-C1-NRN1 green fluorescent protein fusion vector was transfected into 293 cells,and NRN1 localization was assessed using immunofluorescence.Endogenous NRN1 localization was also examined in hippocampal HT22 cells.Results According to the HPA database,NRN1 expression was enriched in the cerebral cortex,parathyroid gland,adipose tissue,and placenta.Experimental validation confirmed NRN 1 expression in all the aforementioned tissues,with highest levels observed in the brain and spleen.Immunofluorescence analysis revealed that NRN 1 was predominantly localized to the membrane in HT22 cells and to the cyto-plasm in 293 cells.Conclusion NRN 1 is expressed in various human tissues,with notably high expression in the brain.It is primarily localized to the cell membrane and cytoplasm.
