Research progress in gene-editing technology in tumor organoids
10.3969/j.issn.1005-4847.2025.05.010
- VernacularTitle:基因编辑在肿瘤类器官中的研究进展
- Author:
Mengyao LI
1
;
Minli HUANG
;
Peng LI
;
Junke XIE
;
Mengtian GUO
;
Yongbin ZHANG
;
Changhong SHI
Author Information
1. 广州中医药大学科技创新中心,广州 510405;空军军医大学实验动物中心,西安 710032
- Publication Type:Journal Article
- Keywords:
gene-editing;
tumor organoids;
CRISPR/Cas9;
stem cell
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(5):721-729
- CountryChina
- Language:Chinese
-
Abstract:
Organoids have become an important technological platform in cancer research,but simulating the primary tumor tissue structure and function still presents problems.The development of gene-editing technology,especially when combined with tumor organoids,provides a new approach for accurately and comprehensively simulating the in vivo characteristics of tumor models.Introducing specific gene mutations or correcting mutations in tumor organoids through gene-editing technology can allow detailed analysis of the mechanisms of tumor initiation and progression,as well as exploring potential therapeutic targets,accelerating the drug-screening process,and providing new insights for personalized cancer treatment.This article reviews the formation of tumor organoids and the technical aspects of gene-editing strategies,emphasizing their unique applications and prospects in tumor organoids.We also propose that accurately simulating the in vivo microenvironment,promoting the standardization and stability of organoid gene-editing technology,and optimizing the efficiency of gene editing can accelerate the application of organoids in precision medicine research.
