Establishment of an animal model of comorbid anxiety and depression induced by sleep interruption stress based on behavioral experiments
10.3969/j.issn.1005-4847.2025.05.008
- VernacularTitle:基于行为实验的睡眠干扰应激诱发焦虑抑郁共患动物模型的建立
- Author:
Yongzhi ZHAO
1
;
Yiwen ZHANG
;
Yanqin LUO
;
Fang CHEN
;
Yanfei XU
;
Ruile PAN
;
Qi CHANG
;
Mengchao LIU
;
Qinghu HE
;
Ning JIANG
;
Xinmin LIU
Author Information
1. 石河子大学药学院/新疆植物药资源利用教育部重点实验室,新疆石河子 832003;中国医学科学院北京协和医学院药用植物研究所,北京 100193;宁波大学新药技术研究院,浙江宁波 315211
- Publication Type:Journal Article
- Keywords:
sleep interruption;
anxiety-depression;
animal model;
behavior
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(5):695-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the effects of sleep interruption(SI)cycles on emotional behavior in ICR mice,and to establish a mouse model of comorbid anxiety and depression induced by SI.Methods Seventy-two male ICR mice(4～5 weeks old)were divided randomly into a blank group and a model group.Mice in the model group were subjected to SI stress modeling for 1,2,and 3 weeks,respectively.After modeling,emotional behaviors were evaluated using open-field,elevated plus maze,light-dark box,marble-burying,and forced-swimming tests.Serum corticosterone levels were detected by enzyme-linked immunosorbent assay.Results Mice in the model group buried significantly more marbles after 1 week of SI stress,compared with the blank group(P＜0.05).After 2 weeks of stress,mice in the model group also showed a significant decrease in the number of crossings in the light-dark box(P＜0.05)and a significant increase in the number of marbles buried(P＜0.01)compared with the control group.After 3 weeks of stress,mice in the model group showed a significant increase in the number of marbles buried(P＜0.05),a significant decrease in the number of crossings in the light-dark box(P＜0.05),and a significant increase in immobility time in the forced-swim test(P＜0.01).Conclusions ICR mice exhibited significant anxiety-related behaviors after 2 weeks of SI modeling and significant anxiety-and depressive-related behavioral changes after 3 weeks.Three weeks of SI stress can be used to establish a model of comorbid anxiety and depression.
