Effect of Yishen Paidu Formula on inflammatory response in chronic renal failure rats by regulating Calcineurin/NFAT signal pathway
10.3969/j.issn.1000-484X.2025.11.018
- VernacularTitle:益肾排毒方调节Calcineurin/NFAT信号通路对慢性肾衰竭大鼠炎症反应的影响
- Author:
Li FENG
1
;
Bowen PENG
;
Bin PENG
;
Shuangyi ZHU
;
Xue FENG
;
Wei XIONG
;
Xi HU
;
Xiaoling ZHAI
;
Xiaohui SUN
;
Zhi GAO
Author Information
1. 武汉市中医医院肾病科,武汉 430014
- Publication Type:Journal Article
- Keywords:
Yishen Paidu Formula;
Calcineurin;
NFAT;
Chronic renal failure rats;
Inflammatory response
- From:
Chinese Journal of Immunology
2025;41(11):2663-2667
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore effect of Yishen Paidu Formula on inflammatory response in chronic renal failure(CRF)rats and role of Calcineurin/nuclear factor of activated T cell(NFAT).Methods:CRF rat model was constructed,and randomly grouped into model group,low,medium and high doses Yishen Paidu Formula groups,with 10 rats in each group,another 10 rats were fed normally as a blank group;general situation of rats was recorded;urine protein quantification kit was applied to detect 24-hour urine protein level of CRF rats in each group;serum creatinine and urea nitrogen levels of CRF rats were detected;ELISA was applied to detect serum IL-1β,IL-6 and TNF-α levels in CRF rats;pathological changes of renal tissue were observed by HE staining;Western blot was applied to detect expressions of fibroblast growth factor 23(FGF23),Klotho protein,Calcineurin,NFAT protein in renal tissue of CRF rats in each group.Results:Compared with blank group,levels of creatinine,urea nitrogen in serum,24 h urine protein in urine,IL-1β,IL-6,TNF-α in serum,and protein levels of FGF23,Calcineurin,NFAT in kidney tissue were obviously increased in model group,level of Klotho protein was obviously decreased(P<0.05).Compared with model group,levels of creatinine,urea nitrogen in serum,24 h urine protein in urine,IL-1β,IL-6,TNF-α in serum,and protein levels of FGF23,Calcineurin,NFAT in kidney tissue were obviously decreased in low,medium and high doses Yishen Paidu Formula groups,level of Klotho protein was obviously increased,which were more significant with dosage increase(P<0.05).Conclusion:Yishen Paidu Formula may alleviate inflammatory response in CRF rats by inhibiting Calcineurin/NFAT signaling pathway.
