Effect of asiaticoside on cognitive impairment in young epileptic rats by regulating the SIRT1/AMPK signaling pathway
10.3969/j.issn.1000-484X.2025.11.020
- VernacularTitle:积雪草苷调节SIRT1/AMPK信号通路对癫痫幼年大鼠认知障碍的影响
- Author:
Ruiqi JIANG
1
;
Yuan MA
1
;
Youfeng WANG
1
;
Gaiqing SHEN
1
;
Rui ZHAO
1
;
Jiebing LI
1
Author Information
1. 南阳市第一人民医院儿科,南阳 473000
- Publication Type:Journal Article
- Keywords:
Asiaticoside;
SIRT1/AMPK signaling pathway;
Epilepsy;
Cognitive impairment
- From:
Chinese Journal of Immunology
2025;41(11):2676-2681
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of asiaticoside on cognitive impairment in young epileptic rats by regulating SIRT1/AMPK signaling pathway.Methods:Young rats were randomly separated into Sham group,Model group,low-dose asiaticoside(20 mg/kg)group,high-dose asiaticoside(40 mg/kg)group and high-dose asiaticoside+EX527 group.The seizure situation of rats was observed;Morris water maze was applied to test spatial learning and memory abilities;ELISA was applied to detect levels of TNF-α,IL-10,IL-6,MDA,SOD and GSH-Px in rat hippocampal tissue;Nissl staining was applied to observe morphological changes of rat hippocampal tissue;TUNEL staining was applied to detect apoptosis of hippocampal neurons in rats;Western blot was applied to detect expressions of Cleaved-caspase-3,Bax,Bcl-2,SIRT1,p-AMPK and AMPK proteins in rat hippocampal tissue.Results:Compared with Sham group,Model group showed severe tissue damage in hippocampal CA1 region of rats,epilepsy score and seizure duration were obviously increased,incubation period was obviously prolonged,levels of TNF-α,IL-6,MDA,neuronal apoptosis rate,expressions of Cleaved-caspase-3 and Bax proteins in CA1 region of hippocampus were obviously increased,the retention time and number of platform crossings were obviously decreased,levels of IL-10,SOD,GSH-Px,protein expressions of Bcl-2,SIRT1,p-AMPK/AMPK in CA1 region of hippocampus were obviously reduced(P<0.05);compared with Model group,tissue damage in hippocampal CA1 region of rats in low-dose and high-dose asiaticoside groups was obviously reduced,the epilepsy score and seizure duration were obviously decreased,incubation period was obviously shortened,levels of TNF-α,IL-6,MDA,neuronal apoptosis rate,expressions of Cleaved-caspase-3 and Bax proteins in CA1 region of hippocampus were obviously reduced,the retention time and the number of platform crossings were obviously increased,levels of IL-10,SOD,GSH-Px,protein expressions of Bcl-2,SIRT1 and p-AMPK/AMPK in CA1 region of hippocampus were obviously increased(P<0.05);EX527 was able to partially reverse the improve-ment effect of asiaticoside on epileptic young rats.Conclusion:Asiaticoside can reduce inflammatory response,oxidative stress and hippocampal neuronal apoptosis in rats and reduce nerve damage by activating SIRT1/AMPK signaling pathway,thus improving cogni-tive function of young epileptic.
