Research of upregulation of macrophage opsonizing receptors by methionine enkephalin in inhibiting influenza virus infection
10.3969/j.issn.1000-484X.2025.11.007
- VernacularTitle:蛋氨酸脑啡肽上调巨噬细胞调理性受体抑制流感病毒感染的研究
- Author:
Gang WEI
1
;
Wenrui FU
;
Yue CHEN
;
Xiaomeng WANG
;
Yuanlong ZHAO
;
Jing TIAN
Author Information
1. 锦州医科大学附属第一医院,锦州 121000
- Publication Type:Journal Article
- Keywords:
MENK;
Macrophage;
Influenza virus;
Opsonizing receptors;
Proteomics analysis
- From:
Chinese Journal of Immunology
2025;41(11):2596-2601,中插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate immunomodulatory effects of methionine enkephalin(MENK)on macrophages,and to explore effect of opsonizing receptors in anti-influenza virus infection of macrophages.Methods:Potential targets for antiviral effects of MENK on macrophages were explored by network pharmacology.Proteomics analysis was used to identify differentially expressed pro-teins(DEPs)in macrophages of MENK-PR8 and PR8 groups.DEPs were analyzed by bioinformatics,and key factors were verified by qPCR and Western blot.Results:MENK had 85 intersection targets with macrophages and influenza viruses,of which 7 were related to phagosome pathway(mmu04145).A total of 215 DEPs were identified by mass spectrometry,which were highly enriched in phago-some(mmu04145)and interaction of viral proteins with cytokines and cytokine receptors(mmu04061)pathways.qPCR and Western blot showed that Fc gamma receptor(FcγR)and complement receptor(CR3)related to phagosome were highly expressed.Conclu-sion:MENK enhances function of phagocytosis and killing virus by upregulating opsonizing receptors via opioid receptor,suggesting that MENK can serve as an immune modulator or a novel preventive drug for influenza viruses.
