Effect of CB-103 on pyroptosis in oral squamous cell carcinoma cells via the ROS/caspase-3/GSDME signaling pathway
10.12007/j.issn.0258-4646.2025.06.013
- VernacularTitle:CB-103通过ROS/caspase-3/GSDME信号通路对口腔鳞状细胞癌细胞焦亡的影响
- Author:
Hongchao TANG
1
;
Xi ZHENG
;
Lingge ZHANG
;
Leilei YANG
Author Information
1. 郑州市口腔医院口腔颌面外科,郑州 450000
- Publication Type:Journal Article
- Keywords:
CB-103;
oral squamous cell carcinoma;
pyroptosis;
reactive oxygen species
- From:
Journal of China Medical University
2025;54(6):553-558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of CB-103 on pyroptosis in oral squamous cell carcinoma(OSCC)cells via the reactive oxygen species(ROS)/caspase-3/gasdermin-E(GSDME)signaling pathway.Methods The OSCC cell line SCC-7 was treated with varying concentrations of CB-103(0,20,40,60,80,and 100 μg/mL).The proliferation activity of CB-103 cells was measured using cell counting kit-8(CCK-8)assay.The cell migration was assessed using a wound-healing assay.Morphological changes in the cells were observed under an inverted microscope.Western blotting was performed to evaluate the protein expression of GSDME,GSDME-N ter-minal,and cleaved caspase-3.ATP release was measured using an ATP detection kit.The levels of intracellular high-mobility group box 1(HMGB1)and ROS were quantified by immunofluorescence.Results Compared to the control group,CB-103 inhibited the proliferation and migration of SCC-7 cells.Additionally,CB-103 increased ROS levels and upregulated the expression of cleaved caspase-3 protein.Furthermore,CB-103 promoted the cleavage of GSDME and increased the number of GSDME-N terminal fragments(all P＜0.05),thereby inducing pyroptosis in SCC-7 cells.Conclusion CB-103 promotes pyroptosis in OSCC cells by activating the ROS/caspase-3/GSDME pathway.
