Effect of macrophage colony-stimulating factor on the growth and migration of oral squamous cell carcinoma cells
10.12007/j.issn.0258-4646.2025.06.015
- VernacularTitle:巨噬细胞集落刺激因子对口腔鳞状细胞癌细胞生长和迁移的影响
- Author:
Yiwen XU
1
;
Jingying LI
1
;
Linxin LIU
1
;
Yingjiao XU
1
;
Minhai NIE
1
;
Xuqian LIU
1
Author Information
1. 西南医科大学附属口腔医院牙周黏膜病科,四川泸州 646000;西南医科大学口颌面修复重建和再生泸州市重点实验室,四川泸州 646000
- Publication Type:Journal Article
- Keywords:
macrophage colony-stimulating factor;
oral squamous cell carcinoma;
migration;
apoptosis;
proliferation
- From:
Journal of China Medical University
2025;54(6):565-570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of macrophage colony-stimulating factor(MCSF)in oral squamous cell carcinoma(OSCC)and its effects on the proliferation,apoptosis,and migration of OSCC cells.Methods Normal gingival and OSCC tissues were collected,and MCSF protein expression was detected using immunohistochemistry and Western blotting.HSC-4 cells were divided into control(no transfection),shNC(transfection with sequence-free plasmid vector lentivirus),and shMCSF(transfection with silent MCSF plasmid vector lentivirus)groups.The expression of MCSF mRNA and protein in HSC-4 cells was detected using quantitative real-time PCR and Western blotting,respectively.Scratch and Transwell assays were used to detect the migration ability of HSC-4 cells.The TUNEL assay determined the apoptosis ability of HSC-4 cells,while a colony formation assay detected the proliferation ability of HSC-4 cells.Results MCSF was highly expressed in OSCC tissues and HSC-4 cells but weakly expressed in normal gingival tissues and Hacat cells.The migration and proliferation ability of HSC-4 cells in the shMCSF group was lower than that in the shNC group(P＜0.05).The apoptosis ability of HSC-4 cells in the shMCSF group was higher than that in the shNC group(P＜0.05).Conclusion MCSF is upregu-lated in OSCC tissues,promoting cell migration and proliferation,while also reducing the apoptosis of OSCC cells.
