Recombinant attenuated Salmonella carrying the p57 and BLID genes induces ap-optosis in breast cancer cells
10.16303/j.cnki.1005-4545.2024.12.09
- VernacularTitle:携带p57和BLID基因的重组减毒沙门菌诱导乳腺癌细胞凋亡
- Author:
Lixia BAO
1
;
Tianyue GU
;
Qing LIU
;
Fanjie ZHU
;
Xinyuan WANG
;
Xiaochen ZHU
;
Ting-ting LIU
;
Jingyu WANG
;
Yuxin WANG
;
Tianming JIN
;
Dongchao ZHANG
Author Information
1. 天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津 300392
- Publication Type:Journal Article
- Keywords:
p57 gene;
BLID gene;
attenuated Salmonella;
breast cancer;
apoptosis
- From:
Chinese Journal of Veterinary Science
2024;44(12):2564-2571
- CountryChina
- Language:Chinese
-
Abstract:
To construct a recombinant attenuated Salmonella strain carrying the p57 and BLID genes and to evaluate its inhibitory effect on breast cancer cells and apoptosis induction in vitro,the recombinant attenuated Salmonella LH430/pEGFP-p57-BLID was constructed.RT-PCR and Western blot were used to detect the transcription and expression of the recombinant attenuated Salmonella in cancer cells.The CCK-8 method was used to assess the safety of the recombinant at-tenuated Salmonella and its impact on the activity of breast cancer cells.The cell scratch assay was used to analyze the influence of the recombinant attenuated Salmonella on the migratory ability of cancer cells.Flow cytometry and Western blot were used to analyze the effect of the recombinant attenuated Salmonella on the apoptosis of MDA-MB-231 cells.The bands of target genes carried by the recombinant attenuated Salmonella could be amplified by PCR,and these target genes could be transcribed and expressed in MDA-MB-231 cells.The recombinant attenuated Salmonella showed good growth characteristics and genetic stability.Compared with the PBS group,recombi-nant attenuated Salmonella LH430/pEGFP-p57-BLID significantly reduced(P＜0.01)the activity of MDA-MB-231 cells and significantly inhibited(P＜0.01)the migration of MDA-MB-231 cells.Recombinant attenuated Salmonella LH430/pEGFP-p57-BLID can obviously increase the number of apoptosis in MDA-MB-231 cells,significantly upregulate(P＜0.01)the expression of apoptotic protein Bax,and significantly downregulate(P＜0.01)the expression of apoptotic protein Bcl-2.The results showed that the recombinant attenuated Salmonella had an inhibitory effect on breast cancer MDA-MB-231 cells and could induce apoptosis,which laid a foundation for the subsequent study of anti-tumor in vivo.
