Utility of immunochromatography in rapid detection of carbapenemases produced by Enterobacterales strains
10.16718/j.1009-7708.2024.06.005
- VernacularTitle:免疫层析技术在肠杆菌目细菌产碳青霉烯酶快速检测中的应用
- Author:
Renru HAN
1
;
Fen PAN
;
Danan YIN
;
Li DING
;
Yan GUO
;
Shi WU
;
Yang YANG
;
Fangyuan YU
;
Jie JIANG
;
Yingying SHI
;
Fupin HU
;
Hong ZHANG
Author Information
1. 复旦大学附属华山医院抗生素研究所,上海200040
- Publication Type:Journal Article
- Keywords:
carbapenem-resistant Enterobacterales;
carbapenemase;
immunochromatography
- From:
Chinese Journal of Infection and Chemotherapy
2024;24(6):658-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of two immunochromatography kits (lateral flow immunoassay) in detecting carbapenemases in carbapenem-resistant Enterobacterales (CRE).Methods A total of 390 nonduplicate clinical isolates of Enterobacterales were collected and tested.The carbapenemase gene was identified by polymerase chain reaction (PCR).The five common carbapenemases were tested by two immunochromatography kits.Results Overall,213 of the 390 Enterobacterales isolates were CRE and 177 strains were carbapenem-susceptible based on antimicrobial susceptibility testing.PCR assay confirmed that 207 of the 213 CRE strains carried carbapenemase genes,including KPC genotype in 119 strains,NDM genotype in 71 strains,IMP genotype in 9 strains,OXA-48-like genotype in 7 strains,both KPC and NDM genes in 1 strain,while none of the five common carbapenemases in 6 CRE strains.Two immunochromatography kits were used simultaneously to test the production of carbapenemases.The results of the two commercial kits were 100％ consistent with PCR results.The sensitivity and specificity of the two kits were 100％ (95％ CI,97.7％-100％) and 100％ (95％ CI,97.4％-100％) in detection of the five carbapenemases.Conclusions The immunochromatography method is rapid and easy to operate,and can be used to detect the production of carbapenmases in clinical isolates.
