Pan-cancer analysis of FAM110B: prognostic significance and immune implications
10.3760/cma.j.cn112309-20240617-00215
- VernacularTitle:FAM110B在泛癌中的预后价值及免疫相关分析
- Author:
Yuwei LI
1
;
Shuangyan SU
1
;
Bihua WU
1
;
Yunpeng SU
1
;
Le GUO
1
Author Information
1. 大理大学基础医学院微生物与免疫学教研室，大理 671000
- Publication Type:Journal Article
- Keywords:
Biomarkers;
FAM110B;
Pan-cancer analysis;
Pancreatic cancer
- From:
Chinese Journal of Microbiology and Immunology
2025;45(9):773-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of family with sequence similarity 110 member B (FAM110B) and analyze its associations with clinical prognosis, tumor heterogeneity, and immune-related gene expression through a pan-cancer analysis.Methods:TCGA and GTEx databases were used to evaluate the differential expression of FAM110B at mRNA level in pan-cancer and normal tissues. SangerBox platform and TISIDB database were used to analyze the associations of the expression level of FAM110B mRNA with clinical stages, histological grades, and overall survival of patients. The cBioPortal database and the GSCALite platform were used to analyze the genetic variations in the FAM110B gene, and the associations of FAM110B expression with immune regulatory genes, immune checkpoints, tumor mutation burden, microsatellite instability, and anti-cancer drug sensitivity. qRT-qPCR and Western blot were used to detect the expression of FAM110B at mRNA and protein levels in pancreatic cancer, respectively. Independent samples t-test was employed to assess the significance of differences between two groups; Spearman correlation coefficient was used to evaluate the associations between variables; Log-rank test was used for survival analysis. Results:FAM110B was abnormally expressed in a variety of tumors and associated with the overall survival of patients ( P<0.05), with the most significant difference observed in pancreatic cancer ( P<0.001). In vitro experiments verified that FAM110B was highly expressed in PANC-1 cells ( P<0.01), a pancreatic cancer cell line, and its expression level was related to pathological staging and histological grading ( P<0.001). In addition, the expression level of FAM110B mRNA was related to the expression levels of multiple immunomodulatory genes and correlated with tumor mutational burden and microsatellite instability in various tumors ( P<0.05). Conclusions:FAM110B is related to the prognosis, immune regulation, and tumor heterogeneity across multiple cancers, demonstrating promising potential in both basic research and clinical treatment of various cancers.
