Identification and biological characteristics analysis of Rhodococcus erythropolis
10.3760/cma.j.cn112309-20240806-00298
- VernacularTitle:红串红球菌鉴定及其生物学特性分析
- Author:
Deyong ZHU
1
;
Siqi ZHOU
;
Yanping XU
;
Xuefeng WANG
;
Lizhong HAN
;
Shuzhen XIAO
Author Information
1. 云南省文山壮族苗族自治州人民医院医学检验科，文山 663000
- Publication Type:Journal Article
- Keywords:
Rhodococcus erythropolis;
Bloodstream infection
- From:
Chinese Journal of Microbiology and Immunology
2025;45(6):479-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the morphology and molecular biology of Rhodococcus erythropolis isolated from blood culture, and clarify its microbiological characteristics, clinical diagnosis and treatment. Methods:Strain F1069 was isolated and cultured. Then, it was analyzed by morphology, physiological tests, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS), 16S rRNA gene sequencing, and whole-genome sequencing analysis.Results:The colonies of Rhodococcus erythropolis were light yellow, moist, round, and raised, and had neatly-edged margins after being cultured for 48 h. They could turn orange-red after a prolonged cultivation time. The strain was gram-positive bacillus without spores and was negative in acid-fast staining. The strain was identified as Rhodococcus erythropolis by MALDI-TOF MS, and the result was confirmed by 16S rRNA gene sequencing. The F1069 strain contained the RbpA resistance gene and multiple virulence genes. Conclusions:Cases of Rhodococcus erythropolis infection are rare. The diagnosis of such cases depends on the pathogen detection results, especially molecular biology methods. A definitive diagnosis enables rapid guidance for clinical anti-infection treatment.
