HPLC fingerprint of Shaoyao Gancao Keli
10.19778/j.chp.2025.05.007
- VernacularTitle:芍药甘草颗粒HPLC指纹图谱研究
- Author:
Shangren LI
1
;
Kaiting DUAN
;
Ting YAYG
;
Fang WANG
;
Difei HUANG
;
Lingling DUAN
;
Junjie LI
;
Shiyong LIU
Author Information
1. 咸宁市药品质量检验检测所,湖北咸宁 437100
- Publication Type:Journal Article
- Keywords:
Shaoyao Gancao Keli;
HPLC;
fingerprint;
quality control
- From:
Drug Standards of China
2025;26(5):488-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish HPLC fingerprint of Shaoyao Gancao Keli to provide quality control.Methods:Waters Xselect CSH C18 column was used.The mobile phase was acetonitrile-0.05％phosphoric acid solution with gradient elution.The detection wavelength was 232 nm.The column temperature was 35 ℃.The flow rate was 0.8 mL·min-1.The injection volume was 10 μL.10 samples were tested by the method.The similarity of 10 samples were evaluated,and the common peaks were identified.Results:HPLC fingerprint of 17 common peaks was estab-lished.10 common peaks were identified which were gallic acid,albiflorin,paeoniflorin,liquiritin apioside,liquiritin,β-1,2,3,4,6-pentagalloylglucose,isoliquiritin apioside,isoliquiritin,liquiritigenin,glycyrrhizic acid.The similarity of 10 samples was higher than 0.95.Conclusion:The method has good separation,accuracy,re-peatability and stability,and could be used as a standard for quality control of Shaoyao Gancao Keli.
