Design of 8-channel gene analyzer
10.19745/j.1003-8868.2025024
- VernacularTitle:八通道基因分析仪的设计
- Author:
Shi-hong MA
1
;
Yu QIAN
1
;
Song LIANG
1
;
Xia-bin LI
1
;
Jing-jing ZHANG
1
;
Yuan ZHAO
1
;
Wei WANG
1
;
Jian-rong CAO
1
Author Information
1. 德诺杰亿(北京)生物科技有限公司,北京 100176
- Publication Type:Journal Article
- Keywords:
8 channel;
gene analyzer;
gene sequencing;
STM32 SCM
- From:
Chinese Medical Equipment Journal
2025;46(2):24-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design an 8-channel gene analyzer to take the place of the widely used gene analyzer with problems in inconvenient consumable replacement and short storage time of electrophoresis polymer.Methods The 8-channel gene analyzer had its mechanical components composed of an automatic sample loading table,a polymer injection module,a high-voltage temperature control module,an optical module and an integrated U box,its electrical control system made up of a host computer(an embedded computer)and three slave computers(a sampling control board,a polymer injection control board and a high-voltage temperature control board).The automatic sample loading table involved in four motors and transmission systems for x,y,z directions and optical alignment,the transmission systems adopted mainly belt drive mode and the optical alignment motor had its threads with an anti-backlash structure;the polymer injuection module was manipulated by the polymer injection control board,and the polymer block was made of highly transparent acrylic material;the high-voltage temperature control module realized the regulation of electrophoresis voltage and the detection of electrophoresis current by the low-ripple precision high-voltage power supply,and controlled the temperature of the heating furnace by the proportional-integral-differential(PID)algorithm;the optical module consisted of an excitation module and a light-receiving module,which had the base of the reflector made of low expansion coefficient alloy material;the integrated U box had the electrophoresis polymer,capillary array,polymer block and anode buffer in a plastic housing;the host computer had the data acquisition software programmed with C# and C++,and the slave computers were controlled by STM32 SCM.Results The 8-channel gene analyzer had no significant differences with the widely used ABI3500 gene analyzer in resolution,precision accuracy and clinical results.Conclusion The 8-channel gene analyzer gains advantages in consumable replacement and storage time of electrophoresis polymer,and can meet the requirements for gene sequencing.[Chinese Medical Equipment Journal,2025,46(2):24-30]
