Comprehensive Analysis of Differentially Expressed Genes with Abnormal Methylation in Colorectal Cancer
10.11969/j.issn.1673-548X.2025.10.012
- VernacularTitle:综合分析结直肠癌异常甲基化差异表达基因
- Author:
Jiangfen LI
1
;
Xinzhi FANG
1
Author Information
1. 830000 乌鲁木齐,新疆医科大学第四附属医院病理科
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Methylation;
Molecular isoforms;
Clinicopathological factors;
Prognostic
- From:
Journal of Medical Research
2025;54(10):64-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen abnormally expressed genes regulated by methylation in colorectal cancer(CRC),and explore the mechanism of methylation in the progression of CRC.Methods Two gene expression datasets(GSE25070 and GSE20916)and 3 methylation datasets(GSE25062,GSE48684 and GSE77718)were downloaded and analyzed by the Gene Expression Omnibus(GEO)database.Gene function and pathway annotation were conducted through the protein-protein interaction network analysis database.GSE103340 was used to analyze the expression differences of hub genes in different molecular subtypes of CRC.TCGA,cBioPortal data-base and survival curve plotter were used to validate the hub genes and to analyze the relationship between the expression levels of hub genes and the clinicopathological parameters and prognosis of CRC.Results A total 12 upregulated-hypomethylated and 73downregulated-hypermethylated genes were obtained.8hub genes regulated by methylation were verified.KIT downregulation and ITP-KB overexpression were associated with poor prognosis in patients with CRC.Conclusion Through the comprehensive analysis of multiple bioinformatics databases,this study provides a new idea for the epigenetic regulation of CRC methylation,and has a certain hint for molec-ular diagnosis and prognosis.
