Mechanism of Baicalein in Modulating Bronchial Epithelial Inflammatory Factors via the Pyroptosis Pathway
10.11969/j.issn.1673-548X.2025.10.008
- VernacularTitle:黄芩素经细胞焦亡途径调控支气管上皮炎性细胞因子的机制
- Author:
Chunya LU
1
;
Qian SANG
1
;
Jianlong LIN
1
Author Information
1. 450000 郑州大学第一附属医院呼吸与危重症医学科
- Publication Type:Journal Article
- Keywords:
Asthma;
Baicalein;
Pyroptosis;
Inflammatory factor
- From:
Journal of Medical Research
2025;54(10):40-44,57
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of baicalein on the expression of inflammatory factors in bronchial epithelial cell(BEC),and explore the underlying regulatory mechanisms involved.Methods Log-phase 16 HBE cells were categorized into five groups:the normal control group,the toluene diisocyanate(TDI)-stimulated group,and the low-,medium-,and high-dose baica-lein groups.The normal control group was cultured with standard medium,while the TDI-stimulated group was treated with TDI-human serum albumin(TDI-HSA)to establish an asthma-like cell model.Following TDI-HSA stimulation,the low-,medium-,and high-dose baicalein groups were intervened with baicalein at final concentrations of 10,20 and 40μmol/L for48hours.Cell viability was assessed using the CCK-8 assay,and lactate dehydrogenase(LDH)release assays were performed to evaluate the LDH release rate.Flow cytometry was used to determine the rate of pyroptosis.Enzyme-linked immunosorbent assays(ELISA)were conducted to measure the levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and interleukin-18(IL-18)in the culture supernatants.Protein ex-pression levels of NLR family,pyrin domain-containing protein 3(NLRP3),cysteine-dependent aspartate-specific protease 1(caspase-1),Gasdermin D and apoptosis-associated speck-like protein containing a CARD(ASC)were examined via Western blot analysis.Results Compared with the normal control group,the cell viability in the TDI stimulation group decreased(P＜0.05),and the LDH release rate,pyroptosis rate,and the levels of IL-1β,IL-6 and IL-18 in the supernatant all increased(P＜0.05).Com-pared with the TDI-stimulated group,the cell viability in the low-,medium-,and high-dose baicalein groups increased(P＜0.05),while the LDH release rate,pyroptosis rate,and the levels of IL-1β,IL-6 and IL-18 in the supernatant all decreased(P＜0.05),and all in a dose-dependent manner(P＜0.05).Compared with the normal control group,the protein expressions of NLRP3,caspase-1,Gasdermin D and ASC in the TDI-stimulated group were all increased(P＜0.05).Compared with the TDI-stimulated group,the protein expressions of NLRP3,caspase-1,Gasdermin D and ASC in the low-,medium-and high-dose baicalein groups were all decreased(P＜0.05),also in a dose-dependent manner(P＜0.05).Conclusion Baicalein may alleviate TDI-induced the expression of inflammatory factors in BEC by modulating the pyroptosis pathway.Through this mechanism,scutellarin enhances cell viabil-ity and reduces the rate of pyroptosis.
