Mechanism Elucidation of Shugan Formula Regulates Intestinal Flora in Liver Cancer Based on Network Pharmacology
10.11969/j.issn.1673-548X.2025.10.007
- VernacularTitle:基于网络药理学解析舒肝方调控肝癌肠道菌群机制
- Author:
Yiting HUANG
1
;
Ningfang MO
1
;
Jiaxin ZHAO
1
Author Information
1. 530200 南宁,广西中医药大学、广西中医肝脾藏象重点研究室
- Publication Type:Journal Article
- Keywords:
Shugan formula;
Liver cancer;
Intestinal flora;
Network pharmacology;
Molecular docking;
Genomic validation
- From:
Journal of Medical Research
2025;54(10):32-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the molecular mechanism of shugan formula in regulating intestinal flora in liver cancer using network pharmacology.Methods The active components and targets of shugan formula were screened using the TCMSP and BATMAN-TCM databases.Targets associated with liver cancer and intestinal flora were extracted from the GeneCards and OMIM databases.The in-tersection targets between the drug and disease were identified using a Venn diagram.Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment analysis were performed on the intersection targets.Molecular docking technology was em-ployed to validate the binding activities of key components,such as quercetin,kaempferol,with core targets,including TP53 and STAT3.The correlation between targets and the prognosis of liver cancer was assessed through survival analysis.Results KEGG enrichment anal-ysis indicated that shugan formula was primarily enriched in pathways such as cancer pathways.GO enrichment analysis suggested its in-volvement in regulating processes like apoptosis.Molecular docking demonstrated stable binding between key components and core targets.Survival analysis revealed that high expression of tumor protein p53gene(TP53)shortened the overall survival of liver cancer patients,whereas high expression of signal transducer and activator of transcription 3(STAT3)prolonged survival.Conclusion Shugan formula may upregulate STAT3 expression,inhibit TP53 expression,modulate relevant signaling pathways,improve the structure of intestinal flora in liver cancer,and thereby inhibit the proliferation and metastasis of liver cancer cells.
