Mechanism of action of baicalin on renal injury caused by Klebsiella pneumoniae of sheep origin
10.16303/j.cnki.1005-4545.2025.11.20
- VernacularTitle:黄芩苷对羊源肺炎克雷伯菌造成肾损伤的作用机制
- Author:
Shifan CHEN
1
;
Wei FAN
;
Bo ZHANG
;
Yan WANG
;
Xiukai TANG
;
Wei WANG
;
Xinyu ZHANG
;
Fuliang SUN
Author Information
1. 延边大学农学院,吉林延吉 133002
- Publication Type:Journal Article
- Keywords:
baicalin;
Klebsiella pneumoniae;
kidney injury;
network pharmacology;
signaling path-way
- From:
Chinese Journal of Veterinary Science
2025;45(11):2457-2465
- CountryChina
- Language:Chinese
-
Abstract:
Based on network pharmacology,molecular docking technology and experimental valida-tion to explore the therapeutic efficacy and mechanism of action of baicalin(BC)on kidney injury caused by Klebsiella pneumoniae(KP)infection.The inhibitory activity of BC against KP was de-termined by in vitro experiments;a mouse kidney injury model was established,and the therapeu-tic effect was preliminarily verified by ophthalmoscopy and pathological histology;three pro-in-flammatory factors,namely,TNF-α,IL-10,and IL-1β,were detected by ELISA;and the cyber-pharmacology technology was utilized by PubChem,TCMSP,STRING,Cytoscape,AutoDocks and other databases and software to construct the PPI network as well as to perform GO function and KEGG enrichment analyses;and molecular docking technology was used to assess the binding ac-tivity of the drugs to the core targets and to speculate on the signaling pathways of the drug action.The results showed that BC had a better inhibitory effect on KP in the in vitro experiments;path-ological histology showed a significant therapeutic effect of BC;compared with the infected group,the content of pro-inflammatory factors TNF-α,IL-10,and IL-1β in the baicalin treatment group were significantly decreased(P≤0.05).Twenty-four core targets and 11 pathways of action were screened by network pharmacology,and BC docked stably with the acquired core targets TP53,PTGS2,MAPK1,MAPK8,TNF,BCL2,and IGF1 molecules,and it was speculated that BC might exert its antibacterial and anti-inflammatory effects through the signaling pathways of PI3K-Akt,MAPK,HIF-1,and NF-kappa B,etc.This study lays the foundation for further research on the mechanism of action of baicalin on renal injury.
