Identification and genetic analysis of a novel goose astrovirus isolated from Jiangxi Province
10.16303/j.cnki.1005-4545.2025.11.05
- VernacularTitle:新型鹅星状病毒江西株的分离鉴定及遗传进化分析
- Author:
Enfu HUANG
1
;
Qianlang GU
;
Yuxin LIU
;
Jun CHEN
;
Deping SONG
;
Jiangnan HUANG
;
Jia TAN
;
Fanfan ZHANG
Author Information
1. 江西生物科技职业学院,江西南昌 330200
- Publication Type:Journal Article
- Keywords:
gosling gout;
goose astrovirus;
isolation and identification;
genetic evolution
- From:
Chinese Journal of Veterinary Science
2025;45(11):2334-2342
- CountryChina
- Language:Chinese
-
Abstract:
In order to understand the genetic variation and whole genome characteristics of goose as-trovirus(GoAstV)in Jiangxi Province,a strain of GoAstV was successfully isolated from a typical gosling gout case sample and the whole genome sequencing and genetic characteristics of the isola-ted strain were analyzed.The results showed that the JXNC1 strain could be stably passaged on LMH cells and could cause mild cytopathic effects in LMH cells.Sequencing and analysis showed that the full-length genome of the strain was 7 173 bp,and its genetic relationship was the closest to the GZ2301(PP966939)reference strain,belonging to the GoAstV-Ⅱ genotype.The complete genome of JXNC1 strain shared 98.1％-98.8％nucleotide similarity with 22 GoAstV-Ⅱ reference strains,and the amino acid similarity of ORF2 gene was 97.6％-99.6％.At the same time,the analysis results showed that the mutation of the strain mainly occurred in the ORF2 gene,and there were 13 amino acid site mutations,of which T630I was a unique mutation.Animal regression experiments showed that the inoculation of JXNC1 strain could cause urate deposition in the or-gans of goslings,congestion and dilatation of hepatic sinusoids,and small focal necrosis of some hepatocytes.Renal tissue tubular dilatation,renal interstitial connective tissue hyperplasia.The re-sults of this study laid a foundation for accurate prevention and control of the disease.
