Construction of recombinant adenovirus expressing PRRSV NADC30-like GP3 and evaluation of immunogenicity in mice
10.16303/j.cnki.1005-4545.2025.11.02
- VernacularTitle:表达PRRSV NADC30-like GP3重组腺病毒的构建及小鼠免疫效果评价
- Author:
Honghuan LI
1
;
Zexuan WANG
;
Yanjie QIAO
;
Yitao LI
;
Ziwei LIU
;
Zhongchen MA
;
Caidong LIU
;
Yaocheng CUI
;
Zhen WANG
;
Yueli WANG
;
Jihai YI
;
Chuangfu CHEN
Author Information
1. 石河子大学动物科技学院,新疆石河子 832003
- Publication Type:Journal Article
- Keywords:
porcine reproductive and respiratory syndrome virus;
NADC30-like;
GP3;
recombinant adenovirus;
immune effect evaluation
- From:
Chinese Journal of Veterinary Science
2025;45(11):2309-2317
- CountryChina
- Language:Chinese
-
Abstract:
This study constructed a porcine reproductive and respiratory syndrome virus(PRRSV)NADC30-like strain GP3 recombinant adenovirus vector vaccine through in vitro homologous re-combination to explore its immunological efficacy evaluation at the mouse level.Using type 5 ade-novirus as a vector,a recombinant adenovirus expressing PRRSV GP3 protein was prepared and i-dentified in vitro by fluorescence observation,PCR,and Western blot analysis.Immunize mice with recombinant adenovirus and detect humoral and cellular immune responses induced by recombi-nant adenovirus using indirect ELISA and ELISpot methods.The recombinant adenovirus rAdGP3 was identified by enzyme digestion,PCR,fluorescence and Western blot,indicating that the recom-binant adenovirus rAdGP3 was successfully constructed and packaged.After immunizing mice,spe-cific antibodies and neutralizing antibodies were produced,indicating that the recombinant adenovi-rus could elicit strong humoral immunity.ELISpot and lymphocyte proliferation assays showed that the recombinant adenovirus vaccine could stimulate the secretion of IFN-γ-specific T lymphocytes and induce the proliferation of lymphocytes,indicating that the recombinant adenovi-rus could enhance the level of cellular immune response.In this study,rAdGP3 recombinant adeno-virus was successfully constructed and had good immunogenicity at the mouse level,which provid-ed a reference for the development of novel PRRSV vaccines.
