Research progress of antigen specific T cell detection technology
10.3969/j.issn.1000-484X.2025.06.038
- VernacularTitle:抗原特异性T细胞检测技术的研究进展
- Author:
Mingzhe ZENG
1
;
Jiahui DU
1
;
Jiahao HU
1
;
Ziqian HE
1
;
Zeming LE
1
;
Jingyi HUANG
1
;
Zhiqing LI
1
Author Information
1. 中国人民解放军海军军医大学免疫学研究所暨免疫与炎症全国重点实验室,上海 200433
- Publication Type:Journal Article
- Keywords:
Antigen specific T cells;
Cytokine;
Activation-induced markers;
Tetramer technology;
T cell receptor sequencing technology
- From:
Chinese Journal of Immunology
2025;41(6):1490-1496
- CountryChina
- Language:Chinese
-
Abstract:
T cell response plays an important role in anti-viral infection and anti-tumor immunity,and antigen-specific T cell detection is essential for study of T cell response.This article reviews progress of antigen-specific T cell detection technology,including enzyme-linked immunospot(ELISPOT)assay,intracellular cytokine staining(ICS)assay and activation-induced labeling(AIM)assay,which based on detection of cytokine secretion or activation phenotypes of specific T cells after stimulation and reactivation with antigen in vitro.Another class of methods include Tetramer technology based on known epitopes-human leukocyte antigen(HLA)restriction and recently developed single-cell transcriptomes and T-cell antigen receptor(TCR)sequencing technology.Application of the above methods has advanced our understanding of antigen specific T cell response:Strength and duration of the response,subpop-ulation information,epitopes and their associated HLA-restriction,TCR cloning information and transcriptome characteristic.
